Smoothened gene alterations in keratocystic odontogenic tumors
- Zhang Rui†1,
- Peng Li-Ying†1,
- Qu Jia-Fei1,
- Hong Ying-Ying1,
- Chen Feng2Email author and
- Li Tie-Jun1Email author
© Rui et al.; licensee BioMed Central Ltd. 2014
Received: 18 June 2014
Accepted: 29 August 2014
Published: 5 September 2014
It has been widely demonstrated that the hedgehog pathway is strongly associated with basal cell carcinoma of the skin (NBCCS). To assess potential DNA alterations related to keratocystic odontogenic tumors (KCOTs), we sequenced smoothened (SMO) genes in 12 sporadic KCOTs.
Polymerase chain reaction (PCR), capillary electrophoresis and dideoxy chain-termination sequencing were used to examine potential DNA alterations in sporadic KCOTs.
Five alterations in SMO genes were detected. Four of these mutations consisted of two synonymous and three missense mutations; two of which have not been reported to date (c.T776A, c.T1281G).
SMO genes may play an important role in the sonic hedgehog (SHH) pathway and could also be responsible for generating KCOTs and NBCCS. However, their influence on SHH signaling remains to be elucidated.
KeywordsKeratocystic odontogenic tumor (KCOT) Hedgehog (HH) signaling pathway Gene mutation SMO gene
Odontogenic keratocyst (OKC) is an aggressive, cystic jaw lesion with strong growth potential and a high recurrence rate. In recent years, the World Health Organization (WHO) revised its name to keratocystic odontogenic tumor (KCOT). This reclassification is based on its aggressive behavior and high recurrence rate, emphasizing that KCOT is a benign tumor rather than a cyst [1–3]. Although the great majority of keratocysts occur in isolation as single, non-syndromic cysts, they may also present as multiple cysts as a feature of the nevoid basal cell carcinoma syndrome (Gorlin syndrome, OMIM#109400) .
At present, there are many manuscripts that focus on the relationship between KCOT and PTCH1 (patched) gene mutations, demonstrating that PTCH1, the gene responsible for NBCCS, may also play an important role in sporadic KCOTs [5–8]. The PTCH1 gene is a tumor suppressor gene located at 9q22.32 . A study of 14 patients with NBCCS-associated KCOTs and 29 patients with sporadic KCOTs indicated that mutations in transmembrane 2 (TM2) are closely related to the development of sporadic KCOTs .
The hedgehog (HH) signaling pathway is a key regulator of embryonic development, controlling both cellular proliferation and cell fate. Binding of sonic hedgehog (SHH) to its receptor, patched (PTCH1), is believed to relieve normal inhibition by PTCH1 of smoothened (SMO), a seven-span transmembrane protein with homology to a G-protein-coupled receptor .
SMO is a tumor-related gene located at 7q32.3, contains 12 exons spanning approximately 24 kb, and encodes a 787-amino-acid transmembrane glycoprotein . Its receptor is a G protein-coupled receptor that interacts with Patched, an important part of the HH signaling pathway during embryogenesis as well as adulthood [12, 13]. The HH pathway has been demonstrated to play an important role in different development-related cancers [14–18], but the exact mechanism of action has not yet been elucidated. The protein generated by SMO is downstream of PTCH1; that is, the expression of PTCH1 restrains the activation of SMO, and thereby inhibits activation of the HH pathway [19–22]. Recent studies have highlighted the therapeutic value of SMO antagonists for the treatment of HH-linked cancers [22, 23]. SMO, the main activator of the HH pathway may serve as a catalyst during the generation of cysts, and therefore, genetic mutations of SMO are of great importance.
Tumor samples and clinical background
Fourteen KCOT samples with a definite diagnosis were acquired from clinical sources at Peking University School of Stomatology, Oral and Maxillofacial Surgery Department. Diagnoses were based on WHO classification of tumors: pathology and genetics of tumors of the head and neck . All samples were from Chinese patients (eight males and six females). Ages varied from 10 to 58 years, with an average of 29.2 years. Experimental protocols used in this study were reviewed and approved by the Ethics Committee of the Peking University Health Science Center (Peking, China). Informed consent was obtained from all subjects.
DNA isolation and mutation analysis
PCR conditions of SMO exons
Forward primer sequence (5′to 3′)
Reverse primer sequence (5′to 3′)
Annealing temperature (°C)
Sun et al., 2008 
TD60 ~ 50
Wang et al., 2013 
Wang et al., 2013 
Wang et al., 2013 
Wang et al., 2013 
Wang et al., 2013 
Wang et al., 2013 
Wang et al., 2013 
Wang et al., 2013 
Wang et al., 2013 
Wang et al., 2013 
Sun et al., 2008 
Sun et al., 2008 
Experimental data were analyzed using the SPSS ver. 10.0 software and are presented as means ± standard deviation (SD) using statistical methods such as a t-test, analysis of bivariate correlation, etc. A P value <0.05 was considered to indicate statistical significance. Results are representative of two independent experiments.
Results and discussion
Hedgehog (HH) signaling pathway
Hedgehog (HH) is a signal transduction pathway closely related to cell growth and differentiation, and plays a vital role in embryonic development. Mutation or abnormal expression of components of this pathway will lead to various developmental defects and/or tumors. Altered HH signaling is implicated in the development of approximately 20-25% of all cancers, especially those of soft tissues . These findings also suggest that proteins of the HH signaling pathway are predominantly located within the epithelial components of glandular odontogenic cysts (GOCs) and dentigerous cysts (DCs). Therefore, the HH signaling pathway may play an important role in the formation of epithelial lining . The discovery of oncogenic mutations in the HH and mitogen-activated protein kinase (MAPK) pathways in over 80% of ameloblastomas, locally destructive odontogenic tumors of the jaw, was reported in 2014 by genomic analysis of archival material. Mutations in SMO are common in ameloblastomas of the maxilla, whereas BRAF mutations are predominant in tumors of the mandible . In addition, SHH subgroup medulloblastomas are genetically distinct in infants, children and adults . Most meningiomas have simple genomes, with fewer mutations, rearrangements and copy-number alterations than reported in other adult tumors. However, several meningiomas harbor more complex patterns of copy-number changes and rearrangements, including one tumor with chromothripsis. A subset of meningiomas lacking NF2 alterations harbored recurrent oncogenic mutations in AKT1 (p.Glu17Lys) and SMO (p.Trp535Leu), and exhibited immunohistochemical evidence of activation of these pathways . In another study, SMO mutations, which activate the SHH signaling pathway, were identified in ~5% of non-NF2 mutant meningiomas. Collectively, these findings identify distinct meningioma subtypes . Moreover, some findings provide a rationale to explore the use of SMO and BCL2 inhibitors as adjuvant therapy for treatment of DLBCL of the GC type . The expression of SMO in NPC is generally high, whereas expression of PTCH-1 is relatively low. Downregulation of PTCH1 and upregulation of SMO may cause abnormal activation of the HH signaling pathway in NPC, albeit that the genesis and development of NPC may be associated with abnormal activation of HH signaling . The HH signaling pathway is controlled by PTCH1 and SMO, which are located on the target cell’s membrane. As the receptor of sonic hedgehog (SHH), the PTCH1 gene (51 kb) encodes a 12-transmembrane-domain protein (total, 1,447 amino acids). There are two homologous genes in humans, PTCH2 and PTCH1, which negatively regulate SMO, a G-protein-coupled receptor, through SHH. SMO belongs to the G protein-coupled receptor FZ/SMO superfamily, containing a field that crosses the cell membrane seven times. Without a ligand (SHH), PTCH and SMO form an inhibitory compound, restraining signal transduction throughout the entire pathway. However, when SHH binds to PTCH1, SMO escapes from the inhibition by PTCH1, resulting in enhanced expression of downstream target genes, including the GLI superfamily . As shown by numerous researchers, abnormal activation of the HH signaling pathway is closely related to the development of a variety of tumors, and both PTCH1 and SMO play a critical role in this pathway.
The HH signaling cascade is highly conserved and involved in the development of disease throughout evolution. Nevertheless, compared with other pathways, our mechanistic understanding of HH signal transduction is remarkably incomplete. In the absence of ligand, the HH receptor Patched (Ptc), represses the key signal transducer Smoothened (Smo) through an unknown mechanism. HH binding to Ptc alleviates this repression, causing Smo redistribution to the plasma membrane, phosphorylation and subsequent opening of the Smo cytoplasmic tail and Smo oligomerization. However, the order and interdependence of these events are poorly understood. We have mathematically modeled and simulated Smo activation for two alternative modes of activation, with Ptc primarily affecting either Smo localization or phosphorylation. Here, we show that Smo localization to the plasma membrane is sufficient for phosphorylation of the cytoplasmic tail in the presence of Ptc. Using fluorescence cross-correlation spectroscopy (FCCS), we also demonstrated that inactivation of Ptc by HH induces Smo clustering irrespective of Smo phosphorylation. Our observations therefore support a model of HH signal transduction whereby subcellular localization of Smo, and not phosphorylation, is the primary target of Ptc function .
Several studies [34–36] have confirmed that the PTCH1 gene is a tumor suppressor; this suggests that its mutation increases the likelihood of developing cancer, although few reports regarding the relationship between SMO and KCOT have been published.
SMO mutations in 12 sporadic and 19 NBCCS-associated KCOTs
Amino acid definition
dbSNP rs# cluster ID
K1, K2, K4,K5, K6, K7,K8, K10, K11
p. = (194)Glu
K2, K3, K4, K5, K6, K7, K8, K9, K10, K11, K12
p. = (388)Gly
N1, N2, N3, N4, N5, N6, N7, N8, N9, N10, N11, N12, N13, N14, N15, N16, N17, N18, N19,
p. = (388)Gly
The second alteration, a heterozygous mutation from A to G, was located at nucleotide position 862 of exon 3 (Figure 1B) but does not result in an amino acid change at p.G194. The third mutation (c.G1444C, with G/C alleles) was identified in seven tumors (Figure 1C), but does not result in an amino acid change at p.G388. One interesting fact about this synonymous mutation is, with the exception of one sporadic KCOT, all other samples (including sporadic and NBCCS-associated) were identified with this mutation, of which 17 were homozygous and 13 were heterozygous. The frequency of G reached a high of 0.7581, which is far higher than the statistic reported in the single-nucleotide polymorphism (SNP) database curated by the National Centre for Biotechnology Information (NCBI) and the 1000 Genomes Project (minor allele frequency [MAF] = 0.2277). This mutation has been reported in many other cell lines associated with mesothelioma, such as MSTO-211H, NCI-H28, JU77, LO68, NO36, ONE58, and STY51 . Therefore, it is highly likely that this mutation plays a significant role in the occurrence of tumors. However, whether this mutation is related to KCOTs should be determined.
PROVEAN human protein batch result
Protein sequence change
Prediction (cutoff = -2.5)
Prediction (cutoff = 0.05)
The missense mutation (c.T1281G, with T/C alleles) has not been reported previously (Figure 1E). This mutation leads to an amino acid change (p.Val334Gly) located on the first extracellular loop of Smoothened, which is closely associated with the function of SMO. Functional studies on transmembrane regions are restricted by technological conditions, but according to the SIFT software (Table 3), mutation of this amino acid leads to damaging changes in the protein; therefore, we believe that this mutation strongly impacts disease occurrence. Additional studies are required to determine whether this mutation causes functional changes in the SMO gene.
The mode of action between the SMO and PTCH genes has yet to be elucidated, but recent studies have shown that any missing gene in yeast will impose pressure on the cell to compensate, thereby leading to additional genetic mutations . Most SMO gene mutations detected are serious alterations, such as missense and synonymous mutations, while frameshift and nonsense mutations have not been detected. Therefore, we believe that the SMO gene mutation may serve as a driving force in patients with KCOTs. To compensate for the defects caused by the SMO mutation, PTCH1 causes the same signaling pathway to mutate, leading to mutation(s) in the PTCH gene. This conjecture needs further work to confirm. In most cases, people believe that tumor growth is driven by the “latest” cancer cell subsets because they carry most cancer mutations. However, many mutations exist at a low frequency, suggesting that tumors contain many subclones the relationships among which are still unclear. A recent report examining the heterogeneity of breast cancer  showed that stem cells in breast epithelia could differentiate into luminal and basal cells, which constitute the epithelia. Some also believe that breast neoplasms induced through Wnt1 overexpression are derived from this cell type. Paracrine interaction between two cell types, which is driven by signaling molecules, and its short distance, maintains co-existence of two types of pedigree. Only luminal cells can produce Wnt1, whereas basal cells rely on this protein to proliferate. Wnt1 induces the Hras gene in basal cells of breast neoplasms, which may contain a mutation that drives cancer progression, but this mutation has not yet been detected in cancerous cells in the lumen. There is cooperation between basal and luminal cell clones; this cooperation is necessary for Wnt1 to drive two types of cells in tumors. In addition, KCOTs occur during the early stages of dental epithelial formation, during which dental lamina and its residential, epithelium and stroma interact with others during growth, subsequently inducing differentiation. Therefore, we hypothesize that mutation of some genes in the subcutaneous interstitial tissue may impose pressure on epithelial genes to compensate for the defect, and that the SMO mutation may play a critical role in this process. However, additional studies are needed for confirmation.
As we know, the hedgehog signaling pathway is being suggested to be a drug target for cancer therapy for its activation in human cancers [45–47]. Therefore, we think the two newly identified SMO mutations deserve to be further investigated for their therapeutic application in cancer treatment.
Following the analysis of SMO gene mutations and combining our results with similar studies, we conclude that SMO plays an important role in the HH signaling pathway and may be responsible for the development of KCOTs and NBCCS. However, further research focusing on the mechanism of their influence on the SHH signaling pathway is required.
We gratefully acknowledge the patients who participated in this study and Dr. Qian Zhang for her technical assistance. This study was supported by grants from the National Natural Science Foundation of China (nos. 81030018, 30872900 and 81200762).
The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: http://www.textcheck.com/certificate/GilDRH.
- Bhargava D, Deshpande A, Pogrel MA: Keratocystic odontogenic tumour (KCOT)–a cyst to a tumour. Oral Maxillofac Surg. 2012, 16 (2): 163-170.View ArticlePubMedGoogle Scholar
- Nayak MT, Singh A, Singhvi A, Sharma R: Odontogenic keratocyst: what is in the name?. J Nat Sc Biol Med. 2013, 4 (2): 282-285.View ArticleGoogle Scholar
- Li TJ: The odontogenic keratocyst: a cyst, or a cystic neoplasm?. J Dent Res. 2011, 90 (2): 133-142.View ArticlePubMedGoogle Scholar
- Gu XM, Zhao HS, Sun LS, Li TJ: PTCH mutations in sporadic and gorlin-syndrome-related odontogenic keratocysts. J Dent Res. 2006, 85 (9): 859-863.View ArticlePubMedGoogle Scholar
- Guo YY, Zhang JY, Li XF, Luo HY, Chen F, Li TJ: PTCH1 gene mutations in keratocystic odontogenic tumors: a study of 43 Chinese patients and a systematic review. PLoS ONE. 2013, 8 (10): e77305-View ArticlePubMedPubMed CentralGoogle Scholar
- Pan S, Dong Q, Sun LS, Li TJ: Mechanisms of inactivation of PTCH1 gene in nevoid basal cell carcinoma syndrome: modification of the two-hit hypothesis. Clin Cancer Res. 2010, 16 (2): 442-450.View ArticlePubMedGoogle Scholar
- Li TJ, Yuan JW, Gu XM, Sun LS, Zhao HS: PTCH germline mutations in Chinese nevoid basal cell carcinoma syndrome patients. Oral Dis. 2008, 14 (2): 174-179.View ArticlePubMedGoogle Scholar
- Musani V, Sabol M, Car D, Ozretic P, Kalafatic D, Maurac I, Oreskovic S, Levanat S: PTCH1 gene polymorphisms in ovarian tumors: potential protective role of c.3944T allele. Gene. 2013, 517 (1): 55-59.View ArticlePubMedGoogle Scholar
- Hahn H, Wicking C, Zaphiropoulous PG, Gailani MR, Shanley S, Chidambaram A, Vorechovsky I, Holmberg E, Unden AB, Gillies S, Hahn H, Wicking C, Zaphiropoulous PG, Gailani MR, Shanley S, Chidambaram A, Vorechovsky I, Holmberg E, Unden AB, Gillies S, Negus K, Smyth I, Pressman C, Leffell DJ, Gerrard B, Goldstein AM, Dean M, Toftgard R, Chenevix-Trench G, Wainwright B, Bale AE: Mutations of the human homolog of Drosophila patched in the nevoid basal cell carcinoma syndrome. Cell. 1996, 85 (6): 841-851.View ArticlePubMedGoogle Scholar
- Stone DM, Hynes M, Armanini M, Swanson TA, Gu Q, Johnson RL, Scott MP, Pennica D, Goddard A, Phillips H, Noll M, Hooper JE, de Sauvage F, Rosenthal A: The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog. Nature. 1996, 384 (6605): 129-134.View ArticlePubMedGoogle Scholar
- Brastianos PK, Horowitz PM, Santagata S, Jones RT, McKenna A, Getz G, Ligon KL, Palescandolo E, Van Hummelen P, Ducar MD, Raza A, Sunkavalli A, Macconaill LE, Stemmer-Rachamimov AO, Louis DN, Hahn WC, Dunn IF, Beroukhim R: Genomic sequencing of meningiomas identifies oncogenic SMO and AKT1 mutations. Nat Genet. 2013, 45 (3): 285-289.View ArticlePubMedPubMed CentralGoogle Scholar
- Wang C, Wu H, Katritch V, Han GW, Huang XP, Liu W, Siu FY, Roth BL, Cherezov V, Stevens RC: Structure of the human smoothened receptor bound to an antitumour agent. Nature. 2013, 497 (7449): 338-343.View ArticlePubMedPubMed CentralGoogle Scholar
- Ruat M, Hoch L, Faure H, Rognan D: Structure of the smoothened receptor. Med Sci. 2013, 29 (10): 855-860.Google Scholar
- Jiang J, Hui CC: Hedgehog signaling in development and cancer. Dev Cell. 2008, 15 (6): 801-812.View ArticlePubMedGoogle Scholar
- Amakye D, Jagani Z, Dorsch M: Unraveling the therapeutic potential of the Hedgehog pathway in cancer. Nat Med. 2013, 19 (11): 1410-1422.View ArticlePubMedGoogle Scholar
- Yang W, Wang J, Moore DC, Liang H, Dooner M, Wu Q, Terek R, Chen Q, Ehrlich MG, Quesenberry PJ, Neel BG: Ptpn11 deletion in a novel progenitor causes metachondromatosis by inducing hedgehog signalling. Nature. 2013, 499 (7459): 491-495.View ArticlePubMedPubMed CentralGoogle Scholar
- Ren Y, Cowan RG, Migone FF, Quirk SM: Overactivation of hedgehog signaling alters development of the ovarian vasculature in mice. Biol Reprod. 2012, 86 (6): 174-View ArticlePubMedPubMed CentralGoogle Scholar
- Jorgensen TJ, Ruczinski I, Yao Shugart Y, Wheless L, Berthier Schaad Y, Kessing B, Hoffman-Bolton J, Helzlsouer KJ, Kao WH, Francis L, Alani RM, Strickland PT, Smith MW, Alberg AJ: A population-based study of hedgehog pathway gene variants in relation to the dual risk of basal cell carcinoma plus another cancer. Cancer Epidemiol. 2012, 36 (5): e288-e293.View ArticlePubMedPubMed CentralGoogle Scholar
- Yang C, Chen W, Chen Y, Jiang J: Smoothened transduces Hedgehog signal by forming a complex with Evc/Evc2. Cell Res. 2012, 22 (11): 1593-1604.View ArticlePubMedPubMed CentralGoogle Scholar
- Shen F, Cheng L, Douglas AE, Riobo NA, Manning DR: Smoothened is a fully competent activator of the heterotrimeric G protein G(i). Mol Pharmacol. 2013, 83 (3): 691-697.View ArticlePubMedPubMed CentralGoogle Scholar
- Myers BR, Sever N, Chong YC, Kim J, Belani JD, Rychnovsky S, Bazan JF, Beachy PA: Hedgehog pathway modulation by multiple lipid binding sites on the smoothened effector of signal response. Dev Cell. 2013, 26 (4): 346-357.View ArticlePubMedPubMed CentralGoogle Scholar
- Mao J, Fan PH, Ma W, Zhang QQ, Wang B, Fan SJ, Li LH: Down-regulation of Smoothened gene expression inhibits proliferation of breast cancer stem cells. Zhonghua Bing Li Xue Za Zhi / Chinese Journal of Pathology. 2013, 42 (4): 262-266.PubMedGoogle Scholar
- Ruat M, Hoch L, Faure H, Rognan D: Targeting of Smoothened for therapeutic gain. Trends Pharmacol Sci. 2014, 35 (5): 237-246.View ArticlePubMedGoogle Scholar
- Thompson L: World Health Organization classification of tumours: pathology and genetics of head and neck tumours. Ear Nose Throat J. 2006, 85 (2): 74-PubMedGoogle Scholar
- Sun LS, Li XF, Li TJ: PTCH1 and SMO gene alterations in keratocystic odontogenic tumors. J Dent Res. 2008, 87 (6): 575-579.View ArticlePubMedGoogle Scholar
- Zhang X, Harrington N, Moraes RC, Wu MF, Hilsenbeck SG, Lewis MT: Cyclopamine inhibition of human breast cancer cell growth independent of Smoothened (Smo). Breast Cancer Res Treat. 2009, 115 (3): 505-521.View ArticlePubMedGoogle Scholar
- Zhang L, Sun ZJ, Chen XM, Chen Z: Immunohistochemical expression of SHH, PTC, SMO and GLI1 in glandular odontogenic cysts and dentigerous cysts. Oral Dis. 2010, 16 (8): 818-822.View ArticlePubMedGoogle Scholar
- Sweeney RT, McClary AC, Myers BR, Biscocho J, Neahring L, Kwei KA, Qu K, Gong X, Ng T, Jones CD, Varma S, Odegaard JI, Sugiyama T, Koyota S, Rubin BP, Troxell ML, Pelham RJ, Zehnder JL, Beachy PA, Pollack JR, West RB: Identification of recurrent SMO and BRAF mutations in ameloblastomas. Nat Genet. 2014, 46 (7): 722-5.View ArticlePubMedPubMed CentralGoogle Scholar
- ᅟ: Genetic mutations predict SMO inhibitor response in SHH medulloblastoma. Canc discov. 2014, 4 (5): 509-Google Scholar
- Clark VE, Erson-Omay EZ, Serin A, Yin J, Cotney J, Ozduman K, Avsar T, Li J, Murray PB, Henegariu O, Yilmaz S, Günel JM, Carrión-Grant G, Yilmaz B, Grady C, Tanrikulu B, Bakircioğlu M, Kaymakçalan H, Caglayan AO, Sencar L, Ceyhun E, Atik AF, Bayri Y, Bai H, Kolb LE, Hebert RM, Omay SB, Mishra-Gorur K, Choi M, Overton JD, Holland EC: Genomic analysis of non-NF2 meningiomas reveals mutations in TRAF7, KLF4, AKT1, and SMO. Science. 2013, 339 (6123): 1077-1080.View ArticlePubMedPubMed CentralGoogle Scholar
- Kunkalla KLY, Qu C, Leventaki V, Agarwal NK, Singh RR, Vega F: Functional inhibition of BCL2 is needed to increase the susceptibility to apoptosis to SMO inhibitors in diffuse large B-cell lymphoma of germinal center subtype. Ann Hematol. 2013, 92: 777-787.View ArticlePubMedPubMed CentralGoogle Scholar
- Xiao BY, Dang H, Gan JY, Cai Q, Zhang GP, Chang H: Expression of PTCH-1 and SMO mRNA in nasopharyngeal carcinoma. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010, 26 (10): 955-958.PubMedGoogle Scholar
- Alcedo J, Ayzenzon M, Von Ohlen T, Noll M, Hooper JE: The Drosophila smoothened gene encodes a seven-pass membrane protein, a putative receptor for the hedgehog signal. Cell. 1996, 86 (2): 221-232.View ArticlePubMedGoogle Scholar
- Levanat S, Kappler R, Hemmerlein B, Doring P, Musani V, Komar A, Oreskovic S, Pavelic B, Hahn H: Analysis of the PTCH1 signaling pathway in ovarian dermoids. Int J Mol Med. 2004, 14 (5): 793-799.PubMedGoogle Scholar
- Honma M, Ohishi Y, Uehara J, Ibe M, Kinouchi M, Ishida-Yamamoto A, Iizuka H: A novel PTCH1 mutation in a patient of nevoid basal cell carcinoma syndrome. J Dermatol Sci. 2008, 50 (1): 73-75.View ArticlePubMedGoogle Scholar
- Hime GR, Lada H, Fietz MJ, Gillies S, Passmore A, Wicking C, Wainwright BJ: Functional analysis in Drosophila indicates that the NBCCS/PTCH1 mutation G509V results in activation of smoothened through a dominant-negative mechanism. Dev Dyn. 2004, 229 (4): 780-790.View ArticlePubMedGoogle Scholar
- Hu J, Ng PC: SIFT indel: predictions for the functional effects of amino acid insertions/deletions in proteins. PLoS ONE. 2013, 8 (10): e77940-View ArticlePubMedPubMed CentralGoogle Scholar
- Marchler-Bauer A, Lu S, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR: CDD: a conserved domain database for the functional annotation of proteins. Nucleic Acids Res. 2011, 39 (Database issue): D225-D229.View ArticlePubMedGoogle Scholar
- Marchler-Bauer A, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, Geer LY, Geer RC, Gonzales NR, Gwadz M, He S, Hurwitz DI, Jackson JD, Ke Z, Lanczycki CJ, Liebert CA, Liu C, Lu F, Lu S, Marchler GH, Mullokandov M, Song JS, Tasneem A, Thanki N, Yamashita RA, Zhang D, Zhang N, Bryant SH: CDD: specific functional annotation with the conserved domain database. Nucleic Acids Res. 2009, 37 (Database issue): D205-D210.View ArticlePubMedGoogle Scholar
- Marchler-Bauer A, Bryant SH: CD-Search: protein domain annotations on the fly. Nucleic Acids Res. 2004, 32 (Web Server issue): W327-W331.View ArticlePubMedPubMed CentralGoogle Scholar
- Cleary AS, Leonard TL, Gestl SA, Gunther EJ: Tumour cell heterogeneity maintained by cooperating subclones in Wnt-driven mammary cancers. Nature. 2014, 508 (7494): 113-117.View ArticlePubMedPubMed CentralGoogle Scholar
- Lim CB, Prele CM, Cheah HM, Cheng YY, Klebe S, Reid G, Watkins DN, Baltic S, Thompson PJ, Mutsaers SE: Mutational analysis of hedgehog signaling pathway genes in human malignant mesothelioma. PLoS ONE. 2013, 8 (6): e66685-View ArticlePubMedPubMed CentralGoogle Scholar
- Candace E, Carroll SM, Stewart DP, Xiaoxi Ouyang J, Ogden SK: The extracellular loops of Smoothened play a regulatory role in control of Hedgehog pathway activation. Development. 2012, 139 (3): 612-621.View ArticleGoogle Scholar
- Teng X, Dayhoff-Brannigan M, Cheng WC, Gilbert CE, Sing CN, Diny NL, Wheelan SJ, Dunham MJ, Boeke JD, Pineda FJ, Hardwick JM: Genome-wide consequences of deleting any single gene. Mol Cell. 2013, 52 (4): 485-494.View ArticlePubMedPubMed CentralGoogle Scholar
- Onishi H, Katano M: Hedgehog signaling pathway as a therapeutic target in various types of cancer. Cancer Sci. 2011, 102 (10): 1756-1760.View ArticlePubMedGoogle Scholar
- Abidi A: Hedgehog signaling pathway: a novel target for cancer therapy: vismodegib, a promising therapeutic option in treatment of basal cell carcinomas. Indian J Pharmacol. 2014, 46 (1): 3-12.View ArticlePubMedPubMed CentralGoogle Scholar
- Matsushita S, Onishi H, Nakano K, Nagamatsu I, Imaizumi A, Hattori M, Oda Y, Tanaka M, Katano M: Hedgehog signaling pathway is a potential therapeutic target for gallbladder cancer. Cancer Sci. 2014, 105 (3): 272-280.View ArticlePubMedPubMed CentralGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.