Table 1 PCR conditions of SMO exons
From: Smoothened gene alterations in keratocystic odontogenic tumors
Exon | Size(bp) | Forward primer sequence (5′to 3′) | Reverse primer sequence (5′to 3′) | Reference | Annealing temperature (°C) |
|---|---|---|---|---|---|
1 | 391 | GGGCTGCTGCTGCTGCTG | GTCCCCGCCCTCTCCAAAACT | Sun et al., 2008 [25] | TD60 ~ 50 |
2 | 283 | AAGAACTGTCCTGCCCAGATG | CCACTGGACCCTGCCCTATAC | Wang et al., 2013 [12] | 63 |
3 | 305 | AATAATTTGCCAAGCCAGCC | CTTCTGATCATGACCCTTCCC | Wang et al., 2013 [12] | 63 |
4 | 256 | AGGGTCATGATCAGAAGGGTC | AGTATGCAGTAGGGCAGAGCC | Wang et al., 2013 [12] | 63 |
5 | 302 | CTGACTTCTGGGAACCTCCAG | GACAGAAGGTGGGTTACTGGC | Wang et al., 2013 [12] | 63 |
6 | 206 | GTGGCGCAGGTATAGTGACTG | GCCCTATAGGAGCTAGCTGGG | Wang et al., 2013 [12] | 63 |
7 | 175 | GACTCCAGAGCCTTAGGACCC | TCCCTATGGCTAACTTGTCCC | Wang et al., 2013 [12] | 63 |
8 | 196 | AAGCAGTTCTTGGACTGAGCC | CCATCCATTGAATCTGCTGTC | Wang et al., 2013 [12] | 55 |
9 | 379 | AGTTGGAAGCTGCAGTGGG | CAAGGCTGTGCTAGAGGCAG | Wang et al., 2013 [12] | 63 |
10 | 228 | CTCTGGAAAGAATGGCATCG | TTCCAAATAATCTGTGTGCCC | Wang et al., 2013 [12] | 55 |
11 | 215 | AATGGCACTGACTATGGGAGG | CCACTCTTCAGATCCTCTGGG | Wang et al., 2013 [12] | 63 |
12a | 292 | GAGCCAGGGCCCCAGGCTCGT | ACGCTCCCTGTCGGCAAGAGT | Sun et al., 2008 [25] | TD65-56 |
12b | 315 | AGTACCATTCCTCGACTGCCT | GGTATTGGTTCCTCTCTTTCC | Sun et al., 2008 [25] | TD65-56 |