Effects of platelet-rich fibrin and piezosurgery on impacted mandibular third molar surgery outcomes
© Uyanik et al. 2015
Received: 22 April 2015
Accepted: 10 July 2015
Published: 26 July 2015
The aim of this study was the comparision of postoperative outcomes in impacted mandibular third molars that were treated using either platelet-rich fibrin (PRF), a combination of PRF and piezosurgery, or conventional rotatory osteotomy.
Patient and methods
The study included 20 patients; 40 extractions of impacted mandibular third molars were performed. Patients were divided into two main groups. In group A (n = 20), traditional surgery was performed on one side (Group 1, n = 10); traditional surgery was performed, and PRF was administered to the extracted socket on the other side of same patient (Group 2, n = 10). In group B (n = 20), on one side, piezosurgery was used for osteotomy, and PRF was administered (Group 3, n = 10); on the other side of same patient, traditional surgery was performed (Group 4, n = 10). Parameters assessed at baseline for each patient included pain, the number of analgesics taken, trismus, and cheek swelling. These variables were also assessed on postoperative days 1, 2, 3, and 7.
Statistical analysis revealed a significant reduction in postoperative pain (sum of 1st, 2nd, 3rd and 7th days) and trismus (on postoperative day 1) in group 2 (traditional surgery + PRF group), and in postoperative pain, the number of analgesics taken (sum of 1st, 2nd,3rd and 7th days) and trismus (on postoperative day 1) in group 3 (piezosurgery + PRF group) compared to groups 1 and 4 (traditional surgery groups), (p ≤ 0.05). However, swelling on postoperative days 1, 3, and 7 did not differ among the groups (p > 0.05). Only difference was on second day between groups 1–4 and 2–4 (p ≤ 0.05).
The results of our study have shown that the use of PRF with traditional surgery and PRF combined with piezosurgery significantly reduced pain during the postoperative period. In addition, PRF in combination with piezosurgery significantly decreased the number of analgesics taken. Both operations also significantly decreased trismus 24 h after the surgery. As a result of this study, PRF and combination use of PRF and piezosurgery have positive effects in reducing postoperative outcomes after impacted third molar surgery.
KeywordsPlatelet-rich fibrin Piezosurgery Impacted third molar surgery Pain Swelling Trismus
In oral surgery, impacted third molar surgery is one of the most common operations performed by oral and maxillofacial surgeons . After the removal of impacted third molars, at the early postoperative periods, patients generally complain of pain, trismus and swelling which are the complications of this procedure [2, 3]. These inflammatory complications still remain an important factor for patients and surgeons are responsible for developing a strategy to reduce the risk of complications and improve postoperative healing . Many attempts have been made to reduce postoperative outcomes following third molar surgery, including platelet-rich plasma administration , cryotherapy , preoperative and postoperative antibiotics , osteotomy using high or low speed rotary instruments , wound draining , the use of different kinds of flaps , postoperative ice packs , corticosteroids , analgesics  and laser .
Piezoelectric surgery has been proposed as an alternative to rotatory drilling instruments in oral surgery involving osteotomies . Piezoelectric ultrasound osteotomy devices are very efficient when used at complex surgical sites, including soft tissues, nerves, and blood vessels due to their ability to selectively cut, which is effective on mineralized structures [1, 16]. The advantage of ultrasonic instruments is that they reduce trauma to hard tissues thanks to their highly accurate and conservative cutting action, which means that the procedure improves healing process . As noted by Sivolella, such instruments have been employed in a wide range of procedures and surgical interventions, including maxillary sinus elevation, bone harvesting, alveolar crest enlargement, implantology, periodontal and orthognathic maxillofacial surgery, dental exposure and extractions, as well as ear, nose and throat surgery for the removal of cysts and tumours .
Platelet-rich fibrin (PRF) is a second-generation immune and platelet concentrate. PRF accrues all blood sample components supporting healing and immunity on just one fibrin membrane . Dr. Joseph Choukroun was the first to address the application of platelet-rich fibrin in oral and maxillofacial surgery. He used autogenous whole blood to create a PRF clot with the help of a centrifuge . Growth factors (Platelet-Derived Growth Factor (PDGF-ββ),Transforming Growth Factor (TGF-β1), Vascular Endothelial Growth Factor (VEGF), Insulin-Like Growth Factor 1), leukocytic cells and their cytokines (interleukin 1β [IL-1β], IL-6, IL-4, tumor necrosis factor α) are enmeshed within the PRF fibrin matrix . PRF has been used in bone augmentation, angiogenesis, wound healing, and periodontal healing with promising results .
The present study evaluated and compared the effects of PRF, PRF combined with piezoelectric surgery, and rotatory instruments on the postoperative period after surgical mandibular third molar extractions.
Patients and methods
This study was performed at the Near East University Faculty of Dentistry in Nicosia, Cyprus between January 2014 and August 2014. A total of 20 patients (10 female, 10 male) between 19 and 31 years of age participated. There was no statistically difference between groups in mean age, in group A; 22.65 and in group B; 22.35 (p = 0.974, p > 0.05). Bilateral extractions were required for all patients. The selection criteria were as follows: (a) the presence of bilateral, symmetrically oriented, impacted lower third molars requiring extraction for prophylactic reasons; (b) the absence of systemic diseases; (c) no chronic opioid use; (d) age >18 years; (e) non-smoker and non-alcoholic; (f) not pregnant; and (g) no allergy to penicillin or other drugs used during the standardized postoperative therapy. Patients taking antibiotics for a current infection, or who had acute pericoronitis or severe periodontal disease at the time of the operation and if tooth needed sectioning during the surgery, were also excluded.
Per the abovementioned criteria, only patients with bilaterally and vertically impacted lower third molars were included. Moreover, we selected cases with similar surgical conditions with respect to the location of the tooth relative to the jaw, the depth of impaction, the relationship with the ramus and inferior alveolar nerve. All patients required an osteotomy. Extractions of mandibular third molars were of moderate difficulty (class I, level C), and were assessed according to the classification system of Pell and Gregory .
Patients were divided into two main groups. In group A (4 male, 6 female, n = 20), on one side of patient, traditional surgery using burs was performed to Group 1 (traditional surgery, n = 10). Traditional surgery was performed, and PRF was placed in the socket of the extracted tooth on the other side of same patient in Group 2 (traditional surgery + PRF, n = 10). In group B (6 male, 4 female, n = 20), on one side, piezosurgery was used for osteotomy and PRF was administered to Group 3 (piezosurgery + PRF, n = 10), and on the other side of same patient, traditional surgery using burs was performed in Group 4 (traditional surgery, n = 10). Only difference between groups A and B was that we used piezosurgery in group 3. A minimum of 21 days separated the two operations in each patient for the return of parameters to preop-baseline prior to commencing second operation. The selection of processes of which technique to use first on each patient were randomly selected. Gender (χ 2 = 1.6, p = 0.659) and operation side distributions (χ 2 = 0.301, p = 0.960) did not differ among groups (p > 0.05).
Parameters examined in each patient, including pain, number of analgesics taken, trismus, and cheek swelling, were evaluated at baseline (prior to surgery) and on postoperative days 1, 2, 3, and 7. All of the examinations were undertaken at approximately the same time of day and by the same surgeon.
All of the patients were informed regarding the surgical procedure, postoperative time, and possible complications. The protocol design was approved by the local ethics committee (project number, NEU/2014/19–101). All of the participants provided written informed consent.
Preparation of PRF gel
PRF was prepared according to the technique described by Choukroun et al. . Approximately 15 min before surgery, a blood sample was taken without anticoagulant in 10 mL glass-coated plastic tubes that were immediately centrifuged (Elektro-mag M415P) at 3,000 rpm for 10 min (approximately 400 g). The platelet-poor plasma that accumulated at the top of the tubes was discarded. PRF was dissected approximately 2 mm below its contact point with the red corpuscles situated beneath, to include any remaining platelets that may have localized below the junction between the PRF and red corpuscles . For each patient, 10 ml tube produced one PRF clot which was adequate to fill one extracted socket.
All of the patients underwent a radiological examination, including panoramic radiography, and all were treated by the same surgeon and assistant.
In both groups the flap incision was triangular in shape which avoids muscle involvement (Archer flap). Surgery was performed under local anesthesia, using nerve blocking agents in the inferior alveolar, lingual (regional anesthesia) and buccal nerves (infiltration anesthesia), which contained 0.012 mg/mL epinephrine HCl and 40 mg/mL articaine HCl (2 mL Ultracaine D-S Forte Ampul; Sanofi Aventis). All of the third molar extractions were performed by raising a full-thickness mucoperiosteal flap. The surgeon used an identical approach during both surgeries, changing only the instrument used. After mucoperiosteal flap reflection, in groups 1 and 4 (traditional surgery groups), osteotomy was performed using a 1.6 mm round bur mounted on a W&H implanted surgical high-speed handpiece, at 40,000 rpm under abundant irrigation. All parts of the tooth were loosened with a lever and then removed. In Group 2 (traditional surgery and PRF group), osteotomy was performed using a bur; after the tooth was removed, PRF was placed in the extracted socket. In group 3 (piezosurgery and PRF group), osteotomy was performed using a piezoelectric device (EMS-Piezon Master Surgery) with an SL 2 cutting insert. Following removal of the tooth, PRF was placed in the extracted socket.
In all of the cases, the postextraction residual cavity of the impacted third molar was cleaned with sterile physiologic saline solution containing no antibacterial agents; 3–0 silk sutures (4 stitches) were used for wound closure and sutures were removed after 7 days. A gauze pack was pressed against the surgical site for the patient to bite on for 30 min. An icepack was then applied to the surgical area for 6 h immediately following surgery, according to an alternating 15 min on-15 min off schedule. No pharmacologic therapies or antibiotics were administered prior to surgery. Postoperatively, the same postoperative instructions were given to all patients: soft and cold diet for 24 h and they were instructed to take amoxicillin (500 mg) three times per day for 5 days, and to use antiseptic (Povidone-Iodine %7.5) mouthwash three times per day for 7 days. Acetaminophen (500 mg) was prescribed postoperatively, to be taken as required (500 mg every 4–6 h).
Pain was assessed during the postoperative periods using visual analog scales (VAS) ranging from 0 (absence of pain) to 10 (most severe pain) in conjunction with a graphic rating scale . The number of analgesic tablets consumed was also recorded. Trismus was evaluated by measuring the distance between the mesial incisal corners of the upper and lower right incisors during maximum mouth opening as described by Ustun et al. . Swelling was recorded clinically using a modification of the tape measure method described by Gabka and Matsumara [25, 26]. Three preoperative measurements were obtained between the following five reference points: the tragus, soft tissue pogonion, lateral corner of the eye, angle of the mandible, and outer corner of the mouth. Measurements were obtained on postoperative days 1, 2, 3, and 7. The preoperative sum of the three measurements was considered the baseline for that side. The difference between each postoperative measurement and the baseline value indexed the facial swelling and trismus for that day . The time of surgery was considered the period between onset of incision and termination of suturing. All patients were seen on each of the four postoperative days and measurements were always obtained by the same individual, both preoperatively and postoperatively, on days 1, 2, 3, and 7 at approximately the same time of day (these measurements were done for each operation, n = 40).
The Chi-square test was used to compare qualitative variables. To compare differences between more than two independent variables, One-Way ANOVA was employed. If group differences were statistically significant, they were compared bilaterally using Least Significance Difference (LSD) test. If the data were not normally distributed, the Kruskal-Wallis test was used instead of One-Way ANOVA. If group differences were statistically significant, the Mann–Whitney U test was used bilaterally, with the Bonferroni correction also applied. A value of p ≤ 0.05 was taken to indicate statistical significance. Statistical analyses were performed using the R statistical software package (ver. 2.14.0).
All of the patients tolerated the medication well, with no serious complications or side effects. Wound healing was uneventful in all patients. The mean times (minutes) it took for extractions were: 21.42 (traditional surgery = group 1), 21.82 (traditional surgery + PRF = group 2), 29.99 (Piezo + PRF = group 3), 24.24 (traditional surgery = group 4). There was no significant group difference in surgery duration (F = 1.249, p = 0.306) (p < 0.05).
Group comparison of VAS pain scores (added across 7 days) (mm)
Traditional + PRF (G2)
Piezo + PRF (G3)
Total number of analgesic doses in 7 days for groups
Traditional + PRF (G2)
Piezo + PRF (G3)
Average of postoperative trismus after 1 day of surgery (%)
Traditional + PRF (G2)
Piezo + PRF (G3)
Average of trismus at 2nd, 3rd and 7th days after surgery, (%)
2nd postoperative day (mm)
3rd postoperative day
7th postoperative day
Mean ± SD
Mean ± SD
Mean ± SD
20.90 ± 17.83
16.21 ± 16.30
6.75 ± 11.84
Traditional+ PRF (G2)
8.70 ± 10.50
7.00 ± 9.40
2.00 ± 3.52
Piezo + PRF (G3)
7.60 ± 6.85
5.30 ± 7.55
0.80 ± 1.61
19.10 ± 19.45
15.60 ± 15.45
8.70 ± 13.15
Average of swelling at 1st, 2nd, 3rd and 7th days, (%)
1st postoperative day (mm)
2nd postoperative day (mm)
3rd postoperative day
7th postoperative day
Mean ± SD
Mean ± SD
Mean ± SD
Mean ± SD
2.20 ± 1.80
1.66 ± 1.71
1.15 ± 1.33
0.00 ± 0.00
Traditional + PRF (G2)
2.10 ± 1.37
1.40 ± 0.96
0.80 ± 0.63
0.00 ± 0.00
Piezo + PRF (G3)
3.00 ± 1.24
2.30 ± 1.16
1.40 ± 1.00
0.00 ± 0.00
3.70 ± 1.63
3.00 ± 0.81
2.00 ± 0.94
0.00 ± 0.00
This study compared the surgical outcomes (pain, number of analgesics taken, swelling, trismus) after extraction of mandibular third molars using PRF and piezosurgery combined with PRF compared to standard rotating handpiece.
In the present study, the combined use of piezosurgery and PRF significantly decreased the number of analgesics taken. However, when PRF was not combined with piezosurgery, there were no statistically differences in the number of analgesics taken compared to groups that used traditional handpieces (the mean values: 9.4, 5.6, 4.3, 9.5, from group 1–4, respectively). In general, piezosurgery decreased the number of analgesics taken, which was in accordance with Barone et al.  and Goyal et al. . In addition, the mean value of total VAS scores were so close in groups 2 and 3 (25.00, 24.45, respectively). According to this results, in impacted third molar surgery, the combined use of PRF and piezosurgery reduced pain more than the use of PRF after traditional surgery.
VAS has been proven to be a reliable and sensitive method for recording pain after oral surgery procedures which is straightforward to apply and widely used [1, 5, 6, 8–13, 15, 16, 18, 19, 25–27]. There are many authors, indicated in their studies that, using PRF is effective in reducing pain, in their studies, patients were recorded to either have no severe pain, significantly less pain or even no pain [17, 19, 21, 28–30]. Although further studies would be needed to deepen the knowledge of this biomaterial to determinate by which mechanism can it reduce pain.
In the literature there are few studies which show the effect of PRF for the control of pain, swelling, and trismus following the extraction of mandibular third molars. Kim et al.  reported that the use of PRF had no effect on pain following the surgical removal of impacted mandibular third molars and Singh et al.  also reported that PRF had no effect on pain following removal of mandibular third molars (no impaction), similar to that observed by Kim et al. . However, in the present study, PRF significantly reduced pain. In the studies by Kim et al. and Singh et al., all of the patients underwent bilateral removal of impacted third molars during a single appointment. In our study, a minimum of 21 days separated the two operations in each patient for the return of parameters to preop-baseline prior to commencing second operation. In addition, the selection of processes of which technique to use first on each patient were randomly selected. The patients of Kim et al. and Singh et al. might not have been able to distinguish the level of pain on each side. This could be because the patients in both of these studies underwent bilateral removal of the third molars during a single appointment. However, the extent of swelling observed in our study was similar to that observed by Kim et al. .
The extent of trismus was significantly less in the groups treated with PRF (%9.03) and piezosurgery combined with PRF (%9.3), compared to the traditional handpieces used in group 1 (%25.61) and group 4 (%26.16) at the first day visits for postoperative interincisal distance, which was used for the evaluation of trismus.
Various methods have been used to measure facial swelling . Our method was modification of tape measuring method of Gabka and Matsamura which was described by Ustun et al. . Although it is not as accurate as computed tomography (CT) scan or magnetic resonance imaging (MRI) and does not make precise measurements of facial soft tissue volume, it is a noninvasive, simple, cost-effective and timesaving method, which provides us with numeric data for determination of soft tissue contour changes. Our study indicated no significant differences on swelling among the techniques used.
The degree of surgical difficulty was evaluated based on anatomic factors (depth of inclusion and ramus relationship) and the position of the third molar as assessed on radiographic examination. This has been reflected in the classification of Pell and Gregory . In our study all the included mandibular third molars were of moderate difficulty (class I, level C). This condition was considered criteria for inclusion to reduce the risk of confounding factors and to obtain adequate homogeneity between the 2 groups.
Not only anatomical factors but bone cutting, sectioning of tooth, flap design, use of rotary instrument, time taken for surgical procedure and factors associated with operator are also accountable for incidence of complications. In the present study, we excluded the case if tooth needed sectioning during the surgery, flap design was triangular in shape in all extractions, there was no statistical difference between surgery durations, all of the examinations and extractions were done by same surgeon to optimize the homogeneity between the groups. In addition operation side and age distributions between the groups were homogeneous.
The use of PRF, and PRF combined with piezosurgery, significantly reduced pain. In addition, PRF in combination with piezosurgery significantly decreased the number of analgesics taken. Both operations also significantly decreased trismus 24 h after the surgery. As a result of this study, PRF and combination use of PRF and piezosurgery have positive effects in reducing postoperative outcomes after impacted third molar surgery.
- Mantovani E, Arduino PG, Schierano G, Ferrero L, Gallesio G, Mozzati M, et al. A split-mouth randomized clinical trial to evaluate the performance of piezosurgery compared with traditional technique in lower wisdom tooth removal. J Oral Maxillofac Surg. 2014;72(10):1890–7.View ArticlePubMedGoogle Scholar
- Lee CT, Zhang S, Leung YY, Li SK, Tsang CC, Chu CH. Patients’ satisfaction and prevalence of complications on surgical extraction of third molar. Patient Prefer Adherence. 2015;10(9):257–63.Google Scholar
- Gelesko S, Long L, Faulk J, Philips C, Dicus C, White RP. Cryotherapy and topical minocycline as adjunctive measures to control pain after third molar surgery: an exploratory study. J Oral Maxillofac Surg. 2011;69:e324–32.View ArticlePubMedPubMed CentralGoogle Scholar
- Osunde OD, Adebola RA, Omeje UK. Management of inflammatory complications in third molar surgery: a review of the literature. Afr Health Sci. 2011;11(3):530–7.PubMedPubMed CentralGoogle Scholar
- Ogundipe OK, Ugboko VI, Owotade FJ. Can autologous platelet-rich plasma gel enhance healing after surgical extraction of mandibular third molars? J Oral Maxillofac Surg. 2011;69:2305–10.View ArticlePubMedGoogle Scholar
- Laureano Filho JR, De Oliveira e Silva ED, Batista CI, Gouveia FM. The influence of cryotherapy on swelling, pain and trismus after third-molar extraction. J Am Dent Assoc. 2005;136(6):774–8.View ArticlePubMedGoogle Scholar
- Olurotimi AO, Gbotolorun OM, Ibikunle AA, Emeka CI, Arotiba GT, Akinwande JA. A comparative clinical evaluation of the effects of preoperative and postoperative antimicrobial therapy on postoperative sequelae after ımpacted mandibular third molar extraction. J Oral Maxillofac Res. 2014;5(2):e2.PubMedPubMed CentralGoogle Scholar
- Barone A, Marconcini S, Giacomelli L, Rispoli L, Calvo JL, Covani U. A randomized clinical evaluation of ultrasound bone surgery versus traditional rotary ınstruments in lower third molar extraction. J Oral Maxillofac Surg. 2010;68:330–6.View ArticlePubMedGoogle Scholar
- Koyuncu BO, Zeytinoğlu M, Tetik A, Gomel MM. Effect of tube drainage compared with conventional suturing on postoperative discomfort after extraction of impacted mandibular third molars. Br J Oral Maxillofac Surg. 2015;53(1):63–7.View ArticlePubMedGoogle Scholar
- Sandhu A, Sandhu S, Kaur T. Comparision of two different flap design in the surgical removal of bilateral impacted mandibular third molars. Int J Oral Maxillofac Surg. 2010;39:1091–6.View ArticlePubMedGoogle Scholar
- Forouzanfar T, Sabelis A, Ausems S, Baart JA, van der Waal I. Effect of ice compression on pain after mandibular third molar surgery: a single-blind, randomized controlled trial. Int J Oral Maxillofac Surg. 2008;37:824–30.View ArticlePubMedGoogle Scholar
- Bamgbose BO, Akinwande JA, Adeyemo WL, Ladeinde AL, Arotiba GT, Ogunlewe MO. Effects of co-administered dexamethasone and diclofenac potassium on pain, swelling and trismus following third molar surgery. Head Face Med. 2005;1:11.View ArticlePubMedPubMed CentralGoogle Scholar
- Pouchain EC, Costa FWG, Bezerra TP, Soares ECS. Comparative efficacy of nimesulide and ketoprofen on inflammatory events in third molar surgery: a split-mouth, prospective, randomized,double-blind study. Int J Oral Maxillofac Surg. 2015; doi:https://doi.org/10.1016/ijoms.2014.10.026.
- Romeo U, Libotte F, Palaia G, Tenore G, Galanakis A, Annibali S. Is Er:YAG laser vs conventional rotary osteotomy better in the post operative period for lower third molar surgery? Randomized split mouth clinical study. Oral Maxillofac Surg. 2015;73(2):211–8.View ArticleGoogle Scholar
- Mozzati M, Gallesio G, Russo A, Staiti G, Mortellaro C. Third-molar extraction with ultrasound bone surgery: a case–control study. J Craniofac Surg. 2014;25:856–9.View ArticlePubMedGoogle Scholar
- Rullo R, Addabbo F, Papaccio G, D’Aquino R, Festa VM. Piezoelectric device vs. conventional rotative instruments in impacted third molar surgery: relationships between surgical difficulty and postoperative pain with histological evaluations. J Craniofac Surg. 2013;41:e33–8.View ArticleGoogle Scholar
- Ruga E, Gallesio C, Boffano P. Platelet-rich fibrin and piezoelectric surgery: a safe technique for prevention of periodontal complications in third molar surgery. J Craniofac Surg. 2011;22(5):1951–5.View ArticlePubMedGoogle Scholar
- Sivolella S, Berengo M, Bressan E, Di Fiore A, Stellini E. Osteotomy for lower third molar germectomy: randomized prospective crossover clinical study comparing piezosurgery and conventional rotatory osteotomy. J Oral Maxillofac Surg. 2011;69:e15–23.View ArticlePubMedGoogle Scholar
- Singh A, Kohli M, Gupta N. Platelet rich fibrin: a novel approach for osseous regeneration. J Maxillofac Oral Surg. 2012;11(4):430–4.View ArticlePubMedPubMed CentralGoogle Scholar
- Hoaglin DR, Lines GK. Prevention of localized osteitis in mandibular third-molar sites using platelet-rich fibrin. Int J Dent. 2013;2013:1–4.View ArticleGoogle Scholar
- Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part IV: Clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:E56–60.View ArticlePubMedGoogle Scholar
- Fragiskos FD. Oral Surgery. Verlag Berlin Heidelberg: Springer; 2007. p. 126.View ArticleGoogle Scholar
- Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:E37–44.View ArticlePubMedGoogle Scholar
- Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part II: Platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:E45–50.View ArticlePubMedGoogle Scholar
- Üstün Y, Erdoğan Ö, Esen E, Karsli ED. Comparison of the effects of 2 doses of methylprednisolone on pain, swelling, and trismus after third molar surgery. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003;96:535–9.View ArticlePubMedGoogle Scholar
- Schultze-Mosgau S, Schmelzeısen R, Frölich JC, Schmele H. Use of Ibuprofen and methylprednisolone for the prevention of pain and swelling after removal of impacted third molars. J Oral Maxillofac Surg. 1995;53:2–7.View ArticlePubMedGoogle Scholar
- Goyal M, Marya K, Jhamb A, Chawla S, Sonoo PR, Singh V, et al. Comparative evaluation of surgical outcome after removal of impacted mandibular third molars using a Piezotome or a conventional handpiece: a prospective study. Br J Oral Maxillofac Surg. 2011;50(6):556–61.View ArticlePubMedGoogle Scholar
- Kumar N, Prasad K, Lalitha RM, Ramanujam L, K R, Dexith J, Chauhan A. Evaluation of treatment outcome after impacted mandibular third molar surgery with the use of autologous platelet rich fibrin: a randomized controlled clinical study. J Oral Maxillofac Surg. 2014; doi:https://doi.org/10.1016/j.joms.2014.11.013.
- Simonpieri A, Del Corso M, Sammartino G, Dohan DM. The relevance of Choukroun’s platelet-rich fibrin and metronidazole during complex maxillary rehabilitations using bone allograft. Part I: a new grafting protocol. Implant Dent. 2009;18:102–11.View ArticlePubMedGoogle Scholar
- Chignon Sicard B, Georgiou CA, Fontas E, David S, Dumas P, Ihrai T, et al. Efficacy of leukocyte- and platelet-rich fibrin in wound healing: a randomized controlled clinical trial. Plast Reconstr Surg. 2012;130(6):819e–29e.View ArticlePubMedGoogle Scholar
- Kim JH, Lee DW, Ryu DM. Effect of platelet-rich fibrin on pain and swelling after surgical extraction of third molars. J Tissue Eng Regen Med. 2011;8(2):80–6.Google Scholar
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