Table 1 Shows an overview of used primary antibody, pretreatment, dilution and exposure time. Footnotes: 1 Leica, Newcastle Upon Tyne, United Kingdom, 2 Agilent/Dako, Glostrup, Denmark, 3 Abcam, Cambridge, United Kingdom, 4 CELL MARQUE, Rocklin, USA
Antigene | Clone | pretreatment | Dilution | Exposure time | Manufactor |
|---|---|---|---|---|---|
CD4 | Novocastra tm Liquid Mouse Monoclonal Antibody CD4 Product Code: NCL-L-CD4-368 | heat, ph 9 | 1:10 | 25 min | Leica1 |
CD8 | FLEX Monoclonal Mouse Anti-Human CD8 Clone c8/144B | heat, ph 9 | undiluted | 25 min | Agilent/Dako2 |
FoxP3 | anti-FOXP3 antibody [236A/E7] ab20034 | heat, ph 9 | 1:50 | 25 min | Abcam3 |
PD-1 | MRQ-22; | heat, ph 9 | undiluted | 20 min | CELL MARQUE4 |