Fig. 3

A TEM images of bone cells at cold temperatures. The osteocytes (O) in the lacunae (L) of the bone at 4 °C (A), 3 °C (B), and (C) 2 °C show acute damages. Some are necrotic with empty lacunae, whereas others vacuolized (arrowhead), shrank, and lost attachment (arrow) to the bone matrix (M). Each picture shows the most representative osteocyte of the respective sample. B TEM images of bone cells at warm temperatures. The osteocytes (O) in the lacunae (L) of the bone at 50 °C (A), (B) 49 °C, and (C) 48 °C show acute damages with necrosis, shrinkage, detachment, and vacuolization, similarly to those treated with cold temperatures. The degree of damage was worst at 50 °C, where almost all osteocytes were gone. Each image shows the most representative osteocyte of the respective sample