Fig. 7

IBA-1 expression in microglia subject to CB receptor agonist modulation with or without CII for 6 h. Immunocytochemical analyses for IBA-1 (green) after stimulation for 6 h in cultured Embryonic stem cell-derived microglia (ESdM) cells. The IBA-1 signals were distributed equally in the cytoplasm for controls and with endocannabinoid treatment (arrowheads on Fig. a, b, c). CII induced loss of cells. IBA-1 MFI values increased in the CII and the CII plus AEA group (arrowheads on Fig. d, e). In the specimes exposed to CII plus PEA, IBA-1 signals dimished (arrowheads on Fig. f). a: Control, b: AEA-treated (50 μM), c: PEA-treated (50 μM), d: 6 h CII, e: 6 h CII plus AEA-treated (50 μM), f: 6 h CII plus PEA-treated (50 μM). Visualization was performed with Alexa 488 (green) secondary antibody. Nuclei were stained with DAPI (blue). Scale bar is 50 μm