Fig. 5

IBA-1 expression in PDL cells subject to CB receptor agonist modulation with or without CII for 6 h. Immunocytochemical analyses for IBA-1 (green) after 6 h stimulation revealed inherent expression in cultured PDL cells. The IBA-1 signals were distributed equally in the cytoplasm for controls and with or without endocannabinoid treatment (arrowheads on Fig. a, b, c), exhibiting dotted structures. IBA-1 MFI values increased in all groups after CII, although signal distribution concentrated around the nuclei due to changes in cell morphology and loss of cells (arrowheads on Fig. d, f). However, AEA was able to reverse effects aroused by CII, and even intensified IBA-1 signals. a: Control, b: AEA-treated (50 μM), c: PEA-treated (50 μM), d: 6 h CII, e: 6 h CII plus AEA-treated (50 μM), f: 6 h CII plus PEA-treated (50 μM). Visualization was performed with Alexa 488 (green) secondary antibody. Nuclei were stained with DAPI (blue). Scale bar is 50 μm