Fig. 2
From: Epidermal growth factor enhances osteogenic differentiation of dental pulp stem cells in vitro
Morphological analysis and expression of mesenchymal stem cell (MSC) markers on dental pulp stem cells (DPSCs). a–d Morphological changes after 7 days of osteoblast differentiation. DPSCs begin to lose the typical spindle-shape MSC morphology and become osteoblast-like cells. Original magnification 10x, scale bar =100 μm. e–h Stemness biomarkers were analyzed by immunocytochemistry. Representative immunofluorescence images show changes in CD146 surface marker expression on DPSCs after osteogenic induction for 1 week. i–l Expression levels of the CD10 marker. Cells were stained with primary antibodies: mouse anti-human CD146-FITC, mouse anti-human CD10-FITC. Original magnification 40x, scale bar =50 μm