Table 1 This table presents the structure of the primers used, their base pair lengths and the corresponding annealing temperatures.
From: Analysis of expression profiles of MAGE-A antigens in oral squamous cell carcinoma cell lines
Gene | Sequence (5'→ 3') | Base pairs (bp) | Annealing temp. (°C) |
|---|---|---|---|
β-Actin | for CTACGTCGCCCTGGACTTCGAGC rev GATGGAGCCGCCGATCCACACGG | 384 | 68 |
MAGE-A2 | for AAGTAGGACCCGAGGCACTG rev GAAGAGGAAGAAGCGGTCTG | 236 | 62 |
MAGE-A3 | for CCAAGCAGCACTCAGTAGGAAGG rev GGAAGCTTTGCTGAAGATC | 150 | 51 |
MAGE-A4 | for GAGCAGACAGGCCAACCG rev AAGGACTCTGCGTCAGGC | 445 | 65 |
MAGE-A6 | for GGAAGGTGGCCAAGTTGGTTC rev CCAGCTGCAAGGAATCGGAAG | 149 | 56 |
MAGE-A10 | for CACAGAGCAGCACTGAAGGAG rev CTGGGTAAAGACTCACTGTCTGG | 484 | 60 |