Figure 5
Immunohistochemistry. (a) Cytokeratin AE1/AE3 was positive both in spindle shape cells and epithelial nests. (b) Vimentin was only positive in spindle shape cell component, while it is negative in the epithelial nests. (c) Ki-67 index of the cancer cells was >30%. (d) The matrix of the calcified materials (arrows) showed positive reaction for osteopontin, while cancer cells did not.