Table 2 The primer sequences and PCR conditions
Gene | Primer(5' – 3') | AT bp | Gene | Primer(5' – 3') | AT bp |
|---|---|---|---|---|---|
MMP20 | F: AAGTGCAAACGTGCACTGTC | 68°C | ENAM | F: GAGACTTGACTTGACAGCTCCTAT | 60°C |
Exon 1 | R: GGTTTTCTAGGGCAGAGGAG | 170 | Exon 1 | R: TCTCTAATACTCACCCAATGCC | 413 |
MMP20 | F: ACTACGCTGTAGACGCGTCA | 58°C | ENAM | F: CAAAGACAAGCTAACAAAGTTCAA | 58°C |
Exon 2 | R: CTCTGAATTTGCAAAGACTTG | 318 | Exon 1 -3 | R: GCCCTCTCAAGTGTATTTCTGACA | 735 |
MMP20 | F: GAAAACATGTTCCTTCCGTT | 58°C | ENAM | F: GCAGCTTGAAAACTACCAGATGAT | 58°C |
Exon 3 | R: AGATGGAATCCAAGTACCAC | 201 | Exon 4 e 5 | R: ACTTTGCCTCGATTTGAGAGTTTA | 573 |
MMP20 | F: GAAGGACTCAATCTTGTTGGC | 62°C | ENAM | F: CACTGGGAAGTTCTAAGGTT | 58°C |
Exon 4 | R: CCAGGTTATGGTGAATTGTGC | 196 | Exon 6 | R: AACGGAGTTATCTAGATAAACAAG | 212 |
MMP20 | F: CCTGTGTTGATACTGTTTTTTTC | 60°C | ENAM | F: CAGCCTGAATCACAGCTCTATT | 58°C |
Exon 5 | R: GGGTGGTCATCAAAGAAGG | 234 | Exon 7 | R: TTAAAAGGCAACAGTATTTGGGTA | 513 |
MMP20 | F: CCCGTTACCATTTTGACCAAC | 60°C | ENAM | F: TTATCATTATCGTCTTTGCCCTAT | 58°C |
Exon 6 | R: AATGAGAGTCGGTGGCGTGT | 210 | Exon 8 | R: CCCAGTTTCCCCATTACATT | 567 |
MMP20 | F: GTAAATCAATCATTGATCTTG | 56°C | ENAM | F: TCGAAGGTGGTTTTCTCCTGTGTT | 58°C |
Exon 7 | R: GCCATTTCTTTCTTTGAGGG | 226 | Exon 9 | R: AGCAGGGGCGAATGGATTGT | 157 |
MMP20 | F: GGTGCAGAGTTTTCGTAAAC | 52°C | ENAM | F: AACACCATGGTGGGAAACAAAG | 58°C |
Exon 8 | R: AAATAAAGATAGATAGTAAAAAGG | 232 | Exon 10.1 | R: TTACGTTCCCAAGCAAAGAAGTTC | 573 |
MMP20 | F: CATCTACAACCAGTAAAAACC | 58°C | ENAM | F: ACAGAATAGGCCTTTTTACAGA | 60°C |
Exon 9 | R: GCAAAGCCAAGATTTCTTATG | 223 | Exon 10.2 | R: ATTGGGTTATATTCAGGGTAGAA | 787 |
AMELX | F: GGATTGGTTGTTACAGATGCC | 59°C | ENAM | F: CAAGAAGAACATTTACCCCATCCT | 60°C |
Exon 1 | R: TGGGCCAACTAAAAAGTAAC | 252 | Exon 10.3 | R: CATGCCATAGTTCAAATTCTCACC | 753 |
AMELX | F: TGTGTTTTATGGAGCATTCA | 65°C | ENAM | F: AGCTGGGCTTCAGAAAAATCCAAT | 60°C |
Exon 2 | R: TTACTCACAGGCATGGCAAAAGCTGC | 148 | Exon 10.4 | R: AGATGGTCTTTGCTGTTGCCTCTC | 709 |
AMELX | F: CCTCCCTGTAAAAGCTACCACC | 67°C | ENAM | F: CTCCAATCCAGAAGGCATCCAA | 60°C |
Exon 3 | R: CTTTACAGAGCCCAGGGCATTG | 126 | Exon 10.5 | R: CTCCACCTGGGTCGCTACTCCTAT | 510 |
AMELX | F: GTAGAACTCACATTCTCAGGC | 67°C | KLK4 | F: GCAGCTTTGCAGTCACAAGC | 58°C |
Exon 4e 5 | R: AATGTCTACATACCGGTGGCC | 292 | Exon 1 | R: AGGGACAAAGAGAGGGATGG | 150 |
AMELX | F: GTAGAACTCACATTCTCAGGC | 67°C | KLK4 | F: TGACTGCTCCTGAACCTCTG | 58°C |
Exon 6 | R: GGCTTCAAAATATACTCACCACTTCC | 994 | Exon 2 | R: ATGAGCCTGATATTAGGCCC | 334 |
AMELX | F: CATCTACAACCAGTAAAAACC | 67°C | KLK4 | F: TTCTCCACCCTTCCCTGAGT | 58°C |
Exon7 | R: GCAAAGCCAAGATTTCTTATG | 223 | Exon 3 e 4 | R: TGCCACAAAACTGACCTGCC | 555 |
AMBN | F: ATTGCAGGAGCAGAGATTCC | 58°C | KLK4 | F: GAATTCTGACTCTCCCTCTC | 58°C |
Exon 1 | R: TGGGTGTTAGGCATGTCATC | 395 | Exon 5 | R: GGTCAATTTCATGGGTTCCC | 214 |
AMBN | F: CCTTTATCCCGGTGGTTTTT | 58°C | Amelotin | F: CTGCAGCTAATAACCCACCTAATGA | 58°C |
Exon 2 | R: CGCTTTTGGATTGCAAGACT | 365 | Exon 1 e 2 | R: AATTGACCTTTTACCACGATGGA | 636 |
AMBN | F: CTTCTTCATTCTGCCCAAGC | 58°C | Amelotin | F: GGGCTGGCATTTTTCCACTCTACAT | 58°C |
Exon 3 | R: TGCAGTAGAATTATAAGACAAAGCTC | 385 | Exon 3 | R: TTTTCCCCACTCCCAAACGA | 437 |
AMBN | F: TCCACCTTTCAGTGATGATTTG | 58°C | Amelotin | F: CGAGGCTTCATCTTTATTTACCTTC | 58°C |
Exon 4 | R: TTGTTTTTGTTTTTCCCTGTCA | 376 | Exon 4 | R: CATTTGTGGATATACGCACCC | 306 |
AMBN | F: CTGGCGACAGAGCAAGATTC | 58°C | Amelotin | F: GCAATAGCCCTTGTAGTCGTAC | 58°C |
Exon 5 | R: TCGATTTATTTGGCACGAGA | 370 | Exon 5 | R: GCATGGTCAGTTCTCTGGGTATGTT | 496 |
AMBN | F: TCCTAGCCTCCCTTCCAGAT | 58°C | Amelotin | F: GGCATAGTAGCAGGCAACTGT | 58°C |
Exon 6 | R: TTATGCCTGAAGGCTACGATT | 452 | Exon 6 | R: ACAAAGTACATTGGAAACCTCACAA | 358 |
AMBN | F: TTGGGTCATACCTCCCAAAA | 58°C | Amelotin | F: ATAGATCATAAGGCAGTTTAACATATT | 58°C |
Exon 7–9 | R: TCATGGATAAATGGGACAATGA | 670 | Exon 7 | R: TAGAAAAGTAGCTGGAGAAGTATAATG | 373 |
AMBN | F: TCATGGATAAATGGGACAATGA | 58°C | Amelotin | F: CTCCATCTTTCCATTCCTACCCA | 58°C |
Exon 10–12 | R: CTGAGTCCCATGATCATTTG | 950 | Exon 8 | R: GAGTAAAAATATTCCCTCATGTTGCT | 527 |
AMBN | F: CAGCCAACTTCCTATTCTCCA | 58°C | Amelotin | F: CTAAAGAATGATATGGATGCTCCTAAT | 58°C |
Exon 13 | R: AAAGCAAGAAGGGGACCTACA | 842 | Exon 9 | R: GAGACCAGAATTTGTCTTCACATTGC | 567 |