Oral lichen planus clinical characteristics in Italian patients: a retrospective analysis

Background Oral lichen Planus (OLP) is a chronic inflammatory disease involving skin and mucous membranes. Its etiology is still uncertain whilst an autoimmune mechanism is known to be implicated. OLP is commonly considered a geriatric disease and gender differences in prevalence are clear, whereby females are generally more frequently affected than males more often during the 5th and 6th decades of life. Lesions are symmetrical and bilateral and the buccal mucosa is frequently involved. The risk of malignant transformation is extremely low. This study aims to describe both the clinical characteristics and the prevalence of OLP among a group of patients from Southern Italy. The results of the present study were compared to analogous retrospective studies. Methods Eighty-seven (31 man and 56 woman) cases of OLP were retrospectively reviewed and demographic and clinical data were collected. Data about OLP as clinical forms, oral and extraoral sites involved and Visual Analogue Scale were also recorded. Results The average age of OLP onset was 59.2 years. The most common clinical presentation was the hyperkeratosic type. Symptomatic OLP was noted in 26.8 % of the patiens. The most frequently affected oral sites were buccal mucosa, tongue, gums. The most frequently associated systemic diseases were diabetes, hypertension, C hepatitis and thyroiditis. Only one patient developed a malignant transformation (1.2 %). Conclusions Previous retrospective studies report data partially comparable with our results. Different geographic area, number of enrolled patients and OLP classification criteria may justify the observed differences.


Background
Lichen planus is a quite common chronic inflammatory disease of the skin and mucous membranes, in which the immunological system is believed to play a leading role [1]. It affects all racial groups whit an estimated incidence of 0.5 %-1.5 % [2,3]. The etiology of the oral lichen planus (OLP) remains still unclear, but probably an unknown antigen alters the basal keratinocytes of the oral mucosa making them a target of the cell immune response. CD4 + T and CD8 + T lymphocytes, producing cytokines, interleukin-2 and tumor necrosis factor, induce a chronic inflammatory response and the keratinocytes apoptosis [4]. Variable degrees of ortho or parakeratosis, acanthosis, hypergranulosis and vacuolar degeneration of basal keratinocytes are a consequence of a maturation delay. Moreover, the inflammatory origin of the disease is proved by the marked layered lymphocytic infiltrate ("band-like") immediately underlying the epithelium [5]. The papule is the elementary lesion that characterize the OLP: their confluence can generate white reticular striae (Wickham striae) or plaques [3]. Papular, reticular and plaque-like OLP are considered as hyperkeratosic or "white form", usually no symptomatic. On the contrary, atrophic, erythematosus, erosive and bullous variants of OLP are symptomatic and distinguished as "red form" [6]. The OLP lesions are usually bilaterally/symmetrically localized and the most common involved sites are the buccal mucosa, tongue, gingiva, lips, floor of mouth, palate. Oral lesions may appear weeks or months before the appearance of cutaneous, oesophageal or genital lesions [7,8].
OLP diagnosis should be done by clinical and histological examination: Van der Maij and Van der Wall postulated a list of clinical and histopatological features that could help in the diagnosis formulation [9]. In particular, the clinical features include bilateral oral lesions and reticular lesions; in case of absence of reticular lesions they suggest to use the term "clinically compatible with OLP". The proposed histopathological features include the presence of liquefaction and degeneration of basal cells and band-like infiltrate of lymphocytes and absence of epithelial dysplasia [9]. The absence of epithelial dysplasia is mandatory to distinguish OLP form lichenoid dysplasia, in fact lichenoid dysplasia may share whit OLP the clinical appearance but not the risk of a neoplastic derailment [10]. The rate of malignant transformation of OLP seems to be lowest than lichenoid dysplasia (1.09 vs. 3.20) but an accurate and prolonged follow-up period is also necessary for OLP patients [11].
Although a recent metanalytic review pointed out "the insufficient evidence to support the effectiveness of any specific treatment", a clinical management of OLP patients is often required, above all in symptomatic patients [12]. The treatment of OLP is no curative, in fact its efficacy is related to the symptoms control and ulcers management (especially in erosive OLP). The asymptomatic forms do not warrant management with drugs; they are only supposed to be periodically followed up by clinicians. On the contrary, symptomatic OLP should be treated whit high topical potency corticosteroids, systemic prednisone or immunosuppressive agents like cyclosporine, tacrolimus and thalidomide [13].
The present study aims to describe a cohort of patients from the south of Italy affected by OLP and aims to compare our data with the data from similar case studies reported in Literature.

Methods
This retrospective study analyzed all consecutive patients affected by OLP referred to the Dental Clinic of University of Bari between January 2011 and June 2014.
Demographic and clinical data from recent and remote anamnesis including gender, age, systemic disorders and related medications were collected. Data about OLP clinical forms, oral and extraoral sites involved and a quantification of the initial symptoms obtained through the Visual Analogical Scale (VAS) were also recorded. The VAS is a continuous scale for recording pain intensity; it ranges from 0 (no pain) to 10 (worst pain) [14].
Statistical differences were calculated in the distribution of different types of OLP in men and women using chi-square test while VAS differences, OLP clinical form distribution in sex and age, were calculated with the ANOVA test.
Results were considered statistically significant if p < 0.05. All patients were diagnosed as OLP applying the Van der Meij and Van der Wall criteria (symmetrical distribution of lesions and grid-shaped lesions) and consequential histological examination [9].
Clobetasol propionate ointment in orabase, systemic corticosteroids and topical calcineurin inhibitors were used alone or in combination to treat OLP signs and symptoms.
This study was approved by the local ethical committee (study n. 4743 -Ethical Committee of Policlinico di Bari) and conducted according to the Declaration of Helsinki. All patients signed a informed consent for the treatment of their clinical data and publication.

Results
Eighty-seven patients met the Van der Meij and Van der Wall criteria [9]. In the Tables 1 and 2 are reported the demographical data and comorbidities in OLP patients. The male:female ratio was 1:1.8 (31 men and 56 women). The mean age of the patients was 63.9 years (range 27-93). The average age of onset of the disease was 59.2 years. The mean age at presentation was 56.5 for women and 61.8 for men (p value = 0.03). Sixtyseven patients (77 %) presented one or more concomitant diseases, while the remaining 20 patients (23 %) were in good health and did not take any drug. The more frequent OLP associated pathologies included hypertension (44.8 %, 39 subjects), diabetes (24.1 %, 21 subjects), C hepatitis (active disease and/or patients who received treatment −18.4 %, 16 subjects), thyroiditis (15 %, 13 subjects), psychiatric disorders as depression and anxiety, (10.3 %, 9 subjects), other systemic diseases as heart disease, prostatitis, hypercholesterolemia (31 %, 27 subjects). Six-teen patients were both affected by diabetes and hypertension (Grinspan syndrome) [8]. Four patients (5 %) also presented cutaneous lesions related to lichen planus. Antihypertensive and antidiabetic medications were the most used medications in our cohort of patients, taken alone or in combination. Only 20 OLP patients did not present associated diseases and did not take any drugs or medications.
In Tables 3 are shown  Red forms of OLP were diagnosed in 44.7 % of the women while the remaining 55.3 % reported white forms. Also in men, the most frequent clinical OLP form was the white one (67.7 %) while only one-third (32.3 %) of them reported the red forms. Older patients tends to develop red forms of OLP (mean age at the onset 59.7 years) while the white ones appears in younger patients (mean age at the onset 57.8 years) but no statistical difference was noted (p > 0.05). Mean age of patients at the onset of OLP white forms was 61.1 and 54.6 years for male and female respectively. Mean age of patients at the onset of OLP red forms was 54.1 and 65.3 years for male and female respectively. The mean duration of OLP lesions was 65 months with no significant differences (p > 0.05) between male (62.7) and female (67.4). A total of 46 patients (52.9 %) were symptomatic, 34 (73.9 % of the symptomatic patients) of these subjects showed an atrophic erosive OLP, while the remaining 8 symptomatic patients suffered from hyperkeratosic OLP. VAS was higher in male than in female (3.4 vs. 3.3) with a mean VAS of 3.3 but ANOVA did not show any statistical difference (p > 0.05). OLP lesions were diagnosed in different mucosal areas, coexisting simultaneously. In particular, the buccal mucosa was the most frequent OLP involved oral site (in 56 patients -64.4 %) while the lateral border and dorsal region of the tongue were involved in 31 patients (35.6 %). OLP lesions on gingival mucosa were noted in 20 patients (23 %) while the involvement of hard palate (7 patients, 8 %) fornix (6 patients, 6.9 %), lip mucosa (6 patients, 6.9 %) and soft palate (3 patients, 3.5 %) was observed less frequently.
Only one (1.2 %) male patient (no smoker) developed a squamous cell carcinoma on his left cheek after 5 years from OLP diagnosis. The neoplasm was completely removed (excisional biopsy) and no recurrence was noted after 5 years. None of the remaining 86 patients developed a squamous cell carcinoma.

Discussion
The present study confirms that OLP can be considered a geriatric pathology with a light preponderance among female in 5th and 6th decade. In our cohort of patients, the most frequent associated comorbidity was the hypertension: as often reported in literature, this association can easily explain the nature of oral lesions related to the assumption of antihypertensive drugs [15,16]. On the other hand, the concomitant presence of these diseases could be completely accidental and compatible with the mean age of the patients. Diabetes has been also supposed to play a role in the OLP pathogenesis in fact patients with OLP and diabetes also seem to present more aggressive (atrophic-erosive) oral lesions [17]. This data may suggest the importance of vascular and neurological factors in the OLP pathogenesis. Grinspan syndrome is characterized by three simultaneous symptoms: hypertension, diabetes mellitus and erosive OLP but Lamey et al. [18] supposed that oral lesions were induced by antihypertensive drugs, frequently taken by diabetic patients.
Even if it has been analyzed in many studies, the cause of the association between the OLP and thyroid's pathologies is still unclear. Two studies showed a very high frequency of anti-nuclear antibodies (ANA), gastric parietal cell antibodies (GPCA), autoantibodies against thyroglobulin (TGA), microsomal anti fraction autoantibodies (TMA) in the serum of the patients affected by OLP [19,20]. Other authors suggest that the circulating thyroid antibodies, in patients affected by Hashimoto's thyroiditis, may contribute to trigger specific autoimmune response, to the skin and/or to the oral mucosa, moreover patients suffered from thyroid diseases represents a subset of OLP patients [21,22].
The prevalence of the C hepatitis in our sample was considerably high (18.4 %): association between OLP and HCV still remain a debatable issue but a recent meta-analysis shows an estimate risk 6 times greater of HCV hepatitis in OLP patients [23]. This data supports  an etiopathological association between C hepatitis and OLP in some geographical areas. The immunological imbalance due to virus activity may trigger the OLP pathogenesis, in predisposed patients [24]. According to Carrozzo et al., HLA-DR6 may be responsible for geographical peculiarities of the HCV-OLP association [25]. World Health Organization (WHO) classify OLP as a potentially malignant disorder although the annual malignant transformation is set at a percentage of less than 0.5 % [26,27]. There are no known predictive factors (clinical and histopathological) of malignant transformation of OLP and to date it is not possible to prevent future cancer development from OLP lesions [27].
Oral examination at least once/twice a year still remain the most effective preventive measure in order to early detect the neoplastic derailment of OLP.
Comparing our results whit previous retrospective studies on OLP (Table 4), appears that OLP has a  light predilection for women like as the majority of autoimmune diseases [28][29][30][31][32][33][34][35][36][37][38][39]. The diagnosis is usually made at the 5th decade of life, according to clinical and histopathological criteria. In all analyzed studies, the OLP lesions were bilateral and symmetrical, involving more than one site at the same time: buccal mucosa, gingiva and tongue are the three oral mucosal sites more frequently involved by OLP lesions. Malignant transformation of OLP lesions has been detected in all the analyzed studies in Table 4, exception for two of them [29][30][31]. The average prevalence of malignant transformation in the OLP lesions was 0.95 %, anyways the onset of oral cancer in a cohort of patients affected by OLP is comparable to the onset of cancer in the general population. According to WHO data OLP is considered a lesions with a low potential for malignant transformation. Lesions susceptible to malignant transformation belong to the red forms.

Conclusions
The data about the associations between OLP and systemic disease are diversified. In the majority of the studies there is no significant relevance, on the contrary in this study the percentage of patients affected by disease as diabetes, hypertension, hepatitis C and thyroiditis turns out to be much higher than the prevalence of this disease in the general population. The geriatric age may contribute to the presence of  co-morbidities in OLP patients and probably also their polypharmacolgical treatment play an important role in OLP pathogenesis and chronicization.