Fig. 5

ACC-83 cells were incubated with both the Nrf2 inhibitor ML385 and MG132 for 12 h to observe the proliferation and apoptosis. A Cell viability was determined by CCK8 assay (mean ± SD). All data were obtained from three independent experiments performed in triplicate. B Cells were treated with MG132 in 0µM, 10µM, 40µM and 70µM for 12 h. C Cells were treated with both ML385 and MG132, the data was measured by flow cytometry. D The apoptosis of the group incubated with MG132 was higher than the group incubated with both ML385 and MG132. *P < 0.05,**P < 0.01 versus group treated with MG132