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Fig. 3 | Head & Face Medicine

Fig. 3

From: Influences of cold atmospheric plasma on apoptosis related molecules in osteoblast-like cells in vitro

Fig. 3

Effects of CAP application on the morphology and apoptosis of cultured human osteoblast-like cells at 1 d. a Actin cytoskeleton of osteoblast-like cells, untreated (CAP -) and treated (CAP +) for 60 s. Cytoskeleton and nucleus are stained with FITC conjugated phalloidin (green) and DAPI (blue), respectively, (n = 3). b Apoptotic effects in human osteoblast-like cells after 60 s of CAP treatment displayed by a Vybrant apoptosis assay. Results of flow cytometry analysis of osteoblast-like cell apoptosis after 24 h of treatment with CAP (CAP +) as compared to untreated cells (CAP -). STS preincubated cells (STS +) served as positive control. Flow cytometry was performed after cells were stained with Annexin-FITC and propidium iodide using Vybrant Apoptosis assay kit, (n = 3). * statistical significance to positive control (p < 0.05)

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