Fig. 2

Stimulatory effect of CAP on p53 and APAF-1 levels in human osteoblast-like cells. a Nuclear translocation of p53 after 60 s of CAP treatment at 15 min, 30 min, 60 min as compared to untreated cells (control), (n = 3). b Immunofluorescence staining of p53 after 60 s of CAP treatment at 1 d as compared to untreated cells (control), (n = 3). c Analysis of APAF-1 protein level in total cell lysate after CAP treatment at 15 min, 30 min, 60 min and 1 d using western blot as compared to untreated cells (CAP -). Protein density values were calculated by measurement of the mean gray values for each blot, subsequent inversion and normalization to β-actin, (n = 3). A representative image of one experiment is shown. d Results of the semi-quantitative analysis performed by ImageJ software. Data were expressed as a band intensity relative to control