Fig. 1

SOX2 and OCT4 expression in odontogenic cysts and tumors. AMs showed focal mild-to-moderate SOX2 staining limited to peripheral ameloblast-like cells (Level 1+) (A). SOX2 was undetectable in AOTs (Level 0) (B) and expressed minimally in a few ameloblast-like cells in COC (Level 1+) (C). In contrast, moderate-to-strong SOX2 expression was noted in all layers of odontogenic epithelium in most AFs (Level 4+) (D), DC (Level 3+) (E) and OKC (Level 4+) (F). Two cases of OKC showed Level 2 + immunoreactivity of SOX2 (G). OCT4 expressed exclusively in OKCs. In OCT4 positive cases, the staining was mild (intensity level +) and localized to the upper epithelial layers (Level 1+) (H). In normal dental follicles, rests of odontogenic epithelium expressed SOX2 at moderate (intensity level ++) staining (I). Tonsillar (J) and germinoma (K) specimens were used as positive controls for SOX2 and OCT4, respectively. Both represented the strong (intensity level +++) staining. Negative controls were stained with isotype-matched antibodies (L) and used as intensity level 0. (100X Magnification)