Fig. 8

IBA-1 expression in microglia subject to CB receptor agonist modulation with or without CII for 10 h. Immunocytochemical analyses for IBA-1 (green) after 10 h stimulation in cultured Embryonic stem cell-derived microglia (ESdM) cells. The IBA-1 signals were distributed equally in the cytoplasm for controls and with endocannabinoid treatment (arrowheads on Fig. a, b, c). CII effected loss of cells. IBA-1 signal diminished upon CII treatment (arrowheads on Fig. d showing cell bodies without fluorescence signal in the cytoplasm). CII plus PEA treatment lead to translocation of IBA-1 signal into the nuclei (arrowheads on Fig. f). IBA-1 signal resembled control cells in the CII specimes additionally exposed to AEA (arrowheads on Fig. e). a: Control, b: AEA-treated (50 μM), c: PEA-treated (50 μM), d: 6 h CII, e: 6 h CII plus AEA-treated (50 μM), f: 6 h CII plus PEA-treated (50 μM). Visualization was performed with Alexa 488 (green) secondary antibody. Nuclei were stained with DAPI (blue). Scale bar is 50 μm