Fig. 6

IBA-1 expression in PDL cells subject to CB receptor agonist modulation with or without CII for 10 h. Immunocytochemical analyses for IBA-1 (green) after stimulation for 10 h revealed inherent expression in cultured PDL cells. The IBA-1 signals were distributed equally in the cytoplasm for controls and with endocannabinoid treatment (arrowheads on Fig. a, b, c), exhibiting dotted structures. CII evoked nuclear damage (arrowheads on Fig. d, f), loss of cells and reversal of IBA-1 signals. However, the more intact nuclei were observed in the CIIspecimens additionally treated with AEA. a: Control, b: AEA-treated (50 μM), c: PEA-treated (50 μM), d: 6 h CII, e: 6 h CII plus AEA-treated (50 μM), f: 6 h CII plus PEA-treated (50 μM). Visualization was performed with Alexa 488 (green) secondary antibody. Nuclei were stained with DAPI (blue). Scale bar is 50 μm