Fig. 3

Proliferation of PDL cells and microglia subject to CB receptor agonist modulation in an unstressed versus CII environment. PDL cells (a) and Embryonic stem cell-derived microglia (ESdM) cells (b) were subjected to AEA (50 μM) or PEA (50 μM) treatment for a total incubation time of 72 h. Samples were additionally treated with or without CII for the first 6 h or 10 h of the total incubation time. Unstimulated cells served as control. Readouts were subsequently performed by a WST assay. Values represent the mean ± SEM (n = 6) of the total cell count. Statistical analyses were done with one-way ANOVA for the comparison between groups followed by Bonferroni correction. The level for statistical significance was set at p < 0.05 (* p < 0.05, ** p < 0.01, *** p < 0.001)