Table 2 List of antibodies, source, clone, dilution and antigen retrieval applied in the study
Antibody | Supplier | Catalogue Number | Clone | Mono/Polyclonal | Species | Dilution | Antigen Retrieval |
|---|---|---|---|---|---|---|---|
p16 | CINTec/Roche | 9517 | E6H4 | Mono | Mouse | KIT- | Citrate buffer pH6.0 |
p21 | Merck Millipore | 05–655 | CP 74 | Mono | Mouse | 1:500 | Citrate buffer pH6.0 |
p27 | BD TL | 610241 | 57/Kip1/p27 | Mono | Mouse | 1:1000 | Citrate buffer pH6.0 |
p53 | Dako | M7001 | DO-7 | Mono | Mouse | 1:100 | EDTA pH8.0 |
Hif-1-alpha | BD TL | 610958 | 54/HIF-1a | Mono | Mouse | 1:50 | EDTA pH8.0 |
Glut 1 | Dako | M 7211 | Clone A 35 | Mono | Mouse | 1:40 | EDTA ph8.0 |
Ca IX | Abcam | ab128883 | - | Poly | Rabbit | 1:1000 | Citrate buffer pH6.0 |
XIAP | BD TL | 610716 | 28/hILP/XIAP | Mono | Mouse | 1:50 | Citrate buffer pH6.0 |
Hsp 70 | Invitrogen | 33–3800 | MB-H1 | Mono | Mouse | 1:40 | Citrate buffer pH6.0 |
a-Catenin | BD TL | 610194 | 5/a-Catenin | Mono | Moue | 1:250 | EDTA pH8.0 |
b-Catenin | BD TL | 610153 | 14/beta-Catenin | Mono | Mouse | 1:1000 | EDTA pH8.0 |
g-Catenin | BD TL | 610253 | 15/g-Catenin | Mono | Mouse | 1:1500 | EDTA pH8.0 |
BCL-6 | Dako | M7211 | PG-B6p | Mono | Mouse | 1:50 | Citrate buffer pH6.0 |
Caspase 3 | Invitrogen | 35-1600Z | 43191 | Mono | Mouse | 1:100 | Citrate buffer pH6.0 |
C-kit | Dako | A 4502 | - | Poly | Rabbit | 1:200 | Citrate buffer pH6.0 |
Cyclin D1 | Novocastra | NCL-L-Cyclin D1-GM | P2D11F11 | Mono | Mouse | 1:20 | EDTA pH8.0 |
EGFR | Dako | K 1492 | pharmDX-Kit | Mono | Mouse | KIT | - |