Figure 4
From: In vitro analysis of the cytotoxicity and the antimicrobial effect of four endodontic sealers
The reaction of the PDL fibroblasts to GuttaFlow, Endosequence BC, Pulp Canal Sealer EWT and AH Plus Jet, stained with Phallacidin/DAPI (magnification A, B and D Ć200, C Ć100, bar = 100 Ī¼m) (A-D, upper panels) and with Calcein-AM/Ethidium homodimer II (magnification E, G and H Ć200, F Ć100, bar = 100 Ī¼m) (E-H, lower panels) is shown. DAPI- stains the nucleus blue, and Phallacidin counterstains the cytoplasm green. The intact membrane of live cells is not permeable for Ethidium homodimer II.