Skip to main content

Table 1 E-Rosettes Assay

From: Preparation and properties of the specific anti-influenza virus transfer factor

Adding reagents

Test tube A

Test tube B

Control tube

Peripheral lymphocyte of human

0.05 ml

0.05 ml

0.05 ml

20% of calf serum protein formulated with Hank's solution

0.25 ml

0.25 ml

0.25 ml

Normal TF 6 kD (diluting 100 multiple)

0.25 ml

――

――

Specific TF 6 kD (diluting 100 multiple)

――

0.25 ml

――

0.9% Normal saline(N.S)

――

――

0.25 ml

mixed at 37°C incubating for 1 h, and centrifuged at 2500 g for 10 min, Then discarded supernatant and resuspended pellet.

1% of rat erythrocytes

1 dript

1 dript

1 dript

  1. After mixed and centrifuged at 500 g for 5 min, and then discarded supernatant and resuspended pellet to add one dript of 2.5% aldehyde and mixed to incubate for 15 min at 4°C after then put out to wipe blade, dye with Gimsas and counting.