From: Preparation and properties of the specific anti-influenza virus transfer factor
Adding reagents | Test tube A | Test tube B | Control tube |
---|---|---|---|
Peripheral lymphocyte of human | 0.05 ml | 0.05 ml | 0.05 ml |
20% of calf serum protein formulated with Hank's solution | 0.25 ml | 0.25 ml | 0.25 ml |
Normal TF 6 kD (diluting 100 multiple) | 0.25 ml | ―― | ―― |
Specific TF 6 kD (diluting 100 multiple) | ―― | 0.25 ml | ―― |
0.9% Normal saline(N.S) | ―― | ―― | 0.25 ml |
mixed at 37°C incubating for 1 h, and centrifuged at 2500 g for 10 min, Then discarded supernatant and resuspended pellet. | |||
1% of rat erythrocytes | 1 dript | 1 dript | 1 dript |