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Table 1 DSMZ Cell Culture Data for L-929 cells.

From: Kinetic oxygen measurements by CVC96 in L-929 cell cultures

Cell line: L-929
Cell type: mouse connective tissue fibroblast
Origin: established from the normal subcutaneous areola and adipose tissue of a male C3H/An mouse; used as target in TNF detection assays
Morphology: fibroblasts growing as monolayer
Medium: 90% RPMI 1640 + 10% FBS
Subculture: split confluent cultures 1:5 to 1:10 using trypsin (do not use trypsin/EDTA); after 2–3 days monolayer will be confluent; split 2–3 times a week; seed out initially at about 1.0–2.0 × 106 cells/25 cm2
Incubation: at 37°C with 5% CO2
Doubling time: ca. 21–24 hours
Harvest: ca. 4–8 × 106 cells/25 cm2
Storage: frozen with 70% medium, 20% FBS, 10% DMSO at about 1–2 × 106 cells/ampoule
Mycoplasma: negative in DAPI, microbiological culture, PCR assays
Species: confirmed as mouse with IEF of AST, MDH, PEP B
Cytogenetics: murine hypertriploid karyotype – 61–67, 12–16 centric fusion markers present
Viruses: ELISA: reverse transcriptase negative