Fig. 2

Frontal cut of samples from rabbit calvaria used in the experimental protocol. a: Control specimen without biomaterial placement (sham operated) where it is observed that the epidural space experimentally created is not maintained. b: Experimental specimen with the unilateral insertion of BCP. c and d: Specimen with the bilateral insertion of BCP and β-TCP. e and f: Integration of the hydroxyapatite particles within the bone tissue. At 90 days, the epidural space created was preserved with the biomaterial inserted. Significant osteoconductive capacity was observed for both biomaterials. New mature trabecular bone is highly vascularized with small sections of chondroid tissue (image c, highlighted in yellow). b, c and e: Backscatter electron microscopy; a, d and f: Toluidine blue histology; OC: outer cortex; IC: inner cortex; Dm: dura mater; ShO: Sham operated area; ve: vascular spaces, mc: medullary cavity; Bt: bone tissue; ChT: Chondroid tissue