The effect of low level laser on condylar growth during mandibular advancement in rabbits
© Abtahi et al; licensee BioMed Central Ltd. 2012
Received: 28 September 2011
Accepted: 23 February 2012
Published: 23 February 2012
It has been shown that Low Level Laser (LLL) has a positive effect on bone formation. The aim of this study was to evaluate the effect of low level laser on condylar growth during mandibular advancement in rabbits.
Materials and methods
Continuous forward mandibular advancement was performed in fourteen male Albino rabbits with the mean age of 8 weeks and the mean weight of 1.5 ± 0.5 kg, with acrylic inclined planes. The rabbits were randomly assigned into two groups after 4 weeks. LLL (KLO3: wave length 630 nm) was irradiated at 3 points around the TMJ, through the skin in the first group. The exposure was performed for 3 minutes at each point (a total of 9 minutes) once a day for 3 weeks. The control group was not exposed to any irradiation. The rabbits in both groups were sacrificed after two months and the histological evaluation of TMJ was performed to compare fibrous tissue, cartilage, and new bone formation in condylar region in both groups. Disc displacement was also detected in both groups. Student's t-test, Exact Fisher and Chi square tests were used for the statistical analysis.
The formation of fibrous tissue was significantly lower, while bone formation was significantly greater in lased group as compared with control group. The thickness of cartilage did not differ significantly between two groups.
Irradiation of LLL (KLO3) during mandibular advancement in rabbits, increases bone formation in condylar region, while neither increase in the cartilage thickness nor fibrous tissues was observed.
KeywordsLow level laser rabbit bite jumping condyle
The Class II malocclusion has been called the most frequent skeletal problem in the orthodontic practice [1, 2]. The solution can involve the use of functional or fixed orthodontic appliances, or both . It has been claimed that the most frequent skeletal problem in Class II patients is mandibular retrognathia [4, 5]. In the treatment of Class II malocclusion, capability to alter patients' facial growth is of particular interest, namely by means of functional appliances [6, 7]. The findings from animal and human studies have been accepted as evidence that functional appliances can stimulate condylar [8–10] or mandibular growth,[11, 12] and are able to make changes in the underlying skeletal pattern of the patient. Therefore the success of Class II treatment with mandibular deficiency depends on the ability of functional appliances to encourage condylar growth.
Quantitative histological studies have clarified the time-dependent nature of the adaptive response, indicating that the initial large changes in cartilaginous proliferation are progressively diminished when restoration of functional equilibrium is obtained .
The development of technologies capable of accentuating the growth potential of mandibular cartilage could allow our profession to predictably accelerate the growth phenomena of this tissue. One stimulus capable of improving this process is the application of low-intensity pulsed ultrasound [14, 15].
Recently, low-level laser was used to enhance bone healing after fracture [16, 17], after mandibular distraction osteogenesis,[18, 19] and also for condylar growth stimulation . The results suggest that Low level laser therapy(LLLT) had a positive effect on the percentage of newly formed bone. Better-quality bone sites may allow early healing, thus shortening total treatment time.
Considering the positive effects of LLLT on bone regeneration and the common tendency of shortening treatment period in orthodontics, the aim of the present study was to evaluate the effect of low level laser on condylar growth during mandibular advancement in rabbits. Our hypothesis was that LLLT could increase bone formation during mandibular advancement.
Materials and methods
Following bonding the bite jumper appliance, rabbits were randomly assigned into two groups of seven. In the first group 630 nm low level laser with 10 mw power and a probe diameter of 0.8 mm (KLO3 Mustang2000, Russia) possessing a continuous mode, was irradiated at 3 points around the TMJ, through the skin from the end of the 3rd week after bite jumping . Exposure was performed for 3 minutes  at each point (a total of 9 minutes) once a day for 3 weeks .
Maximum thickness of condylar fibrous tissue.(The number of fibroblasts and collagen bundles were determined in tissue;extensive seperation of fibroblasts by abunant collagen was considered as Fibrosis.)
Minimum thickness of condylar fibrous tissue
Maximum thickness of condylar cartilage
Minimum thickness of condylar cartilage
Maximum thickness of condylar new bone
Minimum thickness of condylar new bone
After the normal distribution of data was confirmed by Kolmogrov-Smirnov test the data were analyzed by Student t-test, Exact Fisher and Chi square tests.
The power calculation for different variables included a follow: maximum condylar fibrous:0.99, minimum condylar fibrous: 0.70, maximum condylar cartilage: 0.35, minimum condylar cartilage: 0.12, maximum new bone: 1, minimum n new bone: 1. The power of our study for bone formation and condylar cartilage wa above 80% which was completely acceptable.
Comparison of lased and control group condyles in different variables (mm)
Max thickness condylar fibrous tissue
Min thickness condylar fibrous tissue
Max thickness condylar cartilage
Min thickness condylar cartilage
Max thickness condylar new bone
Min thickness condylar new bone
In this study we clearly demonstrated the stimulatory effects of 630 nm low level KLO3 laser irradiation on bone formation in condylar region during mandibular advancement in rabbits. The data of this study suggests that newly formed bone was significantly increased by 3 weeks irradiation around TMJ during employing bite jumper appliance.
Rabie et al have shown that the best response of TMJ to mandibular advancement and the highest level of bone formation in the glenoid fossa was detected on day 21, so we started our laser irradiation on the third week .
Histological examination showed no pathological changes such as bone resorption in condylar area, and lower fibrous tissue formation in lased group indicates lower inflammation established in this group. Statistically significant greater amounts of bone were observed in the experimental group which strongly indicates that application of LLL accelerates the maturation of new bone tissue.
Miloro et al found that LLL accelerates the process of bone regeneration in the mandibles during the consolidation phase after distraction osteogenesis as compared with control animals .
Current theories suggest that transcription of certain nuclear proteins, such as a rhodopsin-kinase enzyme may be photosensitive at certain wavelengths and this may be responsible for the accelerated wound healing capabilities of the LLL .
The results of Stein's studies indicate that low-level laser therapy has a biostimulatory effect on human osteoblast-like cells  and it could promote proliferation and maturation of human osteoblasts in vitro . Similar conclusions have been obtained by Dörtbudak about the effect of soft diode lasers on osteoblasts derived mesenchymal cells .
Liu believes that LLL may accelerate the process of fracture repair or increases the callus volume and bone mineral density, in the early stages of fracture healing .
Khadra et al claimed that the application of LLL with a GaAlAs diode laser device can promote bone healing and formation in skeletal defects .
Future studies are warranted with larger numbers of animals. Also, further research is needed to determine the precise cellular and biochemical effects of LLL treatment on both hard and soft tissues.
Regardng the findings of this study LLL may prove efficacious in allowing a shorter period of functional therapy. Irradiation of LLL (KLO3) during mandibular advancement in rabbits, increases bone formation in condylar region, while no increase in the cartilage thickness or fibrous tissues was observed. This would provide great benefit to patients, allowing them to avoid the burdens of a prolonged treatment
This research was financially supported by research vice chancellor of Mashhad University of Medical Sciences.(Code: 88349)
- Sharma JN: Epidemiology of malocclusions and assessment of orthodontic treatment need for the population of eastern Nepal. World J Orthod. 2009, 10 (4): 311-6.PubMedGoogle Scholar
- Celikoglu M, Akpinar S, Yavuz I: The pattern of malocclusion in a sample of orthodontic patients from Turkey. Med Oral Patol Oral Cir Bucal. 2010, 15 (5): e791-6.View ArticlePubMedGoogle Scholar
- Remmer KR, Mamandras AH, Hunter WS, Way DC: Cephalometric changes associated with treatment using the activator, the Frankel appliance, and the fixed appliance. Am J Orthod. 1985, 88: 363-372. 10.1016/0002-9416(85)90063-6.View ArticlePubMedGoogle Scholar
- McNamara JA: Components of class II malocclusion in children 8-10 years of age. Angle Orthod. 1981, 51: 177-202.PubMedGoogle Scholar
- Staley R: Etiology and prevalence of malocclusion. Textbook of Orthodontics. Edited by: Bishara S. 2001, Philadelphia: WB Saunders, 83-96.Google Scholar
- Singh GD, Clark WJ: Localization of mandibular changes in patients with class II division 1 malocclusions treated with twin-block appliances: finite element scaling analysis. Am J Orthod Dentofacial Orthop. 2001, 119 (4): 419-25. 10.1067/mod.2001.113265.View ArticlePubMedGoogle Scholar
- Clark W: Design and management of Twin Blocks: reflections after 30 years of clinical use. J Orthod. 2010, 37 (3): 209-16. 10.1179/14653121043110.View ArticlePubMedGoogle Scholar
- Elgoyhen JC, Moyers RE, McNamara JA, Riolo ML: Craniofacial adaptation of protrusive function in young rhesus monkeys. Am J Orthod. 1972, 62: 469-480. 10.1016/0002-9416(72)90023-1.View ArticlePubMedGoogle Scholar
- Petrovic AG: Mechanisms and regulation of mandibular condylar growth. Acta Morphol Neerl Scand. 1972, 10: 25-34.PubMedGoogle Scholar
- Ruf S, Baltromejus S, Pancherz H: Effective condylar growth and chin position changes in activator treatment: a cephalometric roentgenographic study. Angle Orthod. 2001, 71: 4-11.PubMedGoogle Scholar
- McNamara JA, Hinton RJ, Hoffman DL: Histologic analysis of temporomandibular joint adaptation to protrusive function in young adult rhesus monkeys (Macaca mulatta). Am J Orthod. 1982, 82: 288-298. 10.1016/0002-9416(82)90463-8.View ArticlePubMedGoogle Scholar
- Rodrigues de Almeida M, Castanha Henriques JF, Rodrigues de Almeida R, Ursi W: Treatment effects produced by Frankel appliance in patients with class II, division 1 malocclusion. Angle Orthod. 2002, 72: 418-425.PubMedGoogle Scholar
- Kiliaridis S, Engstrom C, Thilander B: The relationship between masticatory function and craniofacial morphology. I. A cephalometric longitudinal analysis in the growing rat fed a soft diet. Eur J Orthod. 1985, 7: 273-283.View ArticlePubMedGoogle Scholar
- El-Bialy T, El-Shamy I, Graber TM: Growth modification of the rabbit mandible using therapeutic ultrasound: is it possible to enhance functional appliance results?. Angle Orthod. 2003, 73: 631-639.PubMedGoogle Scholar
- Oyonarte R, Zarate M, Rodriguez F: Low-intensity pulsed ultrasound stimulation of condylar growth in rats. Angle Orthod. 2009, 79: 964-970. 10.2319/080708-414.1.View ArticlePubMedGoogle Scholar
- Bashardoust Tajali S, Macdermid JC, Houghton P, Grewal R: Effects of low power laser irradiation on bone healing in animals: a meta-analysis. J Orthop Surg Res. 2010, 5: 1-10.1186/1749-799X-5-1.View ArticlePubMedPubMed CentralGoogle Scholar
- Kazem Shakouri S, Soleimanpour J, Salekzamani Y, Oskuie MR: Effect of low-level laser therapy on the fracture healing process. Lasers Med Sci. 2010, 25: 73-77. 10.1007/s10103-009-0670-7.View ArticlePubMedGoogle Scholar
- Kreisner PE, Blaya DS, Gaiao L, Maciel-Santos ME, Etges A, Santana-Filho M, de Oliveira MG: Histological evaluation of the effect of low-level laser on distraction osteogenesis in rabbit mandibles. Med Oral Patol Oral Cir Bucal. 2010, 15: e616-618.View ArticlePubMedGoogle Scholar
- Miloro M, Miller JJ, Stoner JA: Low-level laser effect on mandibular distraction osteogenesis. J Oral Maxillofac Surg. 2007, 65: 168-176. 10.1016/j.joms.2006.10.002.View ArticlePubMedGoogle Scholar
- Seifi M, Maghzi A, Gutknecht N, Mir M, Asna-Ashari M: The effect of 904 nm low level laser on condylar growth in rats. Lasers Med Sci. 2010, 25: 61-65.View ArticlePubMedGoogle Scholar
- Rabie AB, Zhao Z, Shen G, Hagg EU, Dr O, Robinson W: Osteogenesis in the glenoid fossa in response to mandibular advancement. Am J Orthod Dentofacial Orthop. 2001, 119: 390-400. 10.1067/mod.2001.112875.View ArticlePubMedGoogle Scholar
- Yamaguchi M, Hayashi M, Fujita S, Yoshida T, Utsunomiya T, Yamamoto H, Kasai K: Low-energy laser irradiation facilitates the velocity of tooth movement and the expressions of matrix metalloproteinase-9, cathepsin K, and alpha(v) beta(3) integrin in rats. Eur J Orthod. 2010, 32: 131-139. 10.1093/ejo/cjp078.View ArticlePubMedGoogle Scholar
- Rabie AB, Xiong H, Hagg U: Forward mandibular positioning enhances condylar adaptation in adult rats. Eur J Orthod. 2004, 26: 353-358. 10.1093/ejo/26.4.353.View ArticlePubMedGoogle Scholar
- Scardino M, Swaim S, Sartin E, Hoffman C, Oglivie G, Hanson R, Coolman SL, Davenport E: The effects of omega-3 fatty acid diet enrichment on wound healing. Veterinary Dermatology. 1999, 10: 283-90. 10.1046/j.1365-3164.1999.00148.x.View ArticleGoogle Scholar
- Bliziotes M, Murtagh J, Wiren K: Beta-adrenergic receptor kinase-like activity and beta-arrestin are expressed in osteoblastic cells. J Bone Miner Res. 1996, 11: 820-826.View ArticlePubMedGoogle Scholar
- Stein E, Koehn J, Sutter W, Wendtlandt G, Wanschitz F, Thurnher D, Baghestanian M, Turhani D: Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells. Wien Klin Wochenschr. 2008, 120: 112-117. 10.1007/s00508-008-0932-6.View ArticlePubMedGoogle Scholar
- Stein A, Benayahu D, Maltz L, Oron U: Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg. 2005, 23: 161-166. 10.1089/pho.2005.23.161.View ArticlePubMedGoogle Scholar
- Dortbudak O, Haas R, Mallath-Pokorny G: Biostimulation of bone marrow cells with a diode soft laser. Clin Oral Implants Res. 2000, 11: 540-545. 10.1034/j.1600-0501.2000.011006540.x.View ArticlePubMedGoogle Scholar
- Liu X, Lyon R, Meier HT, Thometz J, Haworth ST: Effect of lower-level laser therapy on rabbit tibial fracture. Photomed Laser Surg. 2007, 25: 487-494. 10.1089/pho.2006.2075.View ArticlePubMedGoogle Scholar
- Khadra M, Kasem N, Haanaes HR, Ellingsen JE, Lyngstadaas SP: Enhancement of bone formation in rat calvarial bone defects using low-level laser therapy. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004, 97: 693-700. 10.1016/j.tripleo.2003.11.008.View ArticlePubMedGoogle Scholar
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